TY - JOUR
T1 - The ER repeat protein YT521-B localizes to a novel subnuclear compartment
AU - Nayler, Oliver
AU - Hartmann, Annette M.
AU - Stamm, Stefan
PY - 2000/9/4
Y1 - 2000/9/4
N2 - The characterization of distinct subnuclear domains suggests a dynamic nuclear framework supporting gene expression and DNA replication. Here, we show that the glutamic acid/arginine-rich domain protein YT521-B localizes to a novel subnuclear structure, the YT bodies. YT bodies are dynamic compartments, which first appear at the beginning of S-phase in the cell cycle and disperse during mitosis. Furthermore, in untreated cells of the human cell line MCF7 they were undetectable and appeared only after drug-induced differentiation. YT bodies contain transcriptionally active sites and are in close contact to other subnuclear structures such as speckles and coiled bodies. YT bodies disperse upon actinomycin D treatment, whereas other transcriptional inhibitors such as α-amanitin or DRB have little effect. On the basis of our experiments, we propose that YT521-B may participate in the assembly of genes into transcription centers, thereby allowing efficient regulation of gene expression.
AB - The characterization of distinct subnuclear domains suggests a dynamic nuclear framework supporting gene expression and DNA replication. Here, we show that the glutamic acid/arginine-rich domain protein YT521-B localizes to a novel subnuclear structure, the YT bodies. YT bodies are dynamic compartments, which first appear at the beginning of S-phase in the cell cycle and disperse during mitosis. Furthermore, in untreated cells of the human cell line MCF7 they were undetectable and appeared only after drug-induced differentiation. YT bodies contain transcriptionally active sites and are in close contact to other subnuclear structures such as speckles and coiled bodies. YT bodies disperse upon actinomycin D treatment, whereas other transcriptional inhibitors such as α-amanitin or DRB have little effect. On the basis of our experiments, we propose that YT521-B may participate in the assembly of genes into transcription centers, thereby allowing efficient regulation of gene expression.
KW - Actinomycin D
KW - Cell cycle
KW - MCF7 differentiation
KW - Subnuclear compartments
KW - Transcription
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U2 - 10.1083/jcb.150.5.949
DO - 10.1083/jcb.150.5.949
M3 - Article
C2 - 10973987
AN - SCOPUS:0034605094
SN - 0021-9525
VL - 150
SP - 949
EP - 961
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 5
ER -