The ER repeat protein YT521-B localizes to a novel subnuclear compartment

Oliver Nayler, Annette M. Hartmann, Stefan Stamm

Research output: Contribution to journalArticlepeer-review

80 Scopus citations


The characterization of distinct subnuclear domains suggests a dynamic nuclear framework supporting gene expression and DNA replication. Here, we show that the glutamic acid/arginine-rich domain protein YT521-B localizes to a novel subnuclear structure, the YT bodies. YT bodies are dynamic compartments, which first appear at the beginning of S-phase in the cell cycle and disperse during mitosis. Furthermore, in untreated cells of the human cell line MCF7 they were undetectable and appeared only after drug-induced differentiation. YT bodies contain transcriptionally active sites and are in close contact to other subnuclear structures such as speckles and coiled bodies. YT bodies disperse upon actinomycin D treatment, whereas other transcriptional inhibitors such as α-amanitin or DRB have little effect. On the basis of our experiments, we propose that YT521-B may participate in the assembly of genes into transcription centers, thereby allowing efficient regulation of gene expression.

Original languageEnglish
Pages (from-to)949-961
Number of pages13
JournalJournal of Cell Biology
Issue number5
StatePublished - Sep 4 2000


  • Actinomycin D
  • Cell cycle
  • MCF7 differentiation
  • Subnuclear compartments
  • Transcription

ASJC Scopus subject areas

  • Cell Biology


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