The ergot alkaloid gene cluster in Claviceps purpurea: Extension of the cluster sequence and intra species evolution

Thomas Haarmann; Caroline Machado; Yvonne Lübbe; Telmo Correia; Christopher L. Schardl; Daniel G. Panaccione; Paul Tudzynski

doi:10.1016/j.phytochem.2005.04.011

The ergot alkaloid gene cluster in Claviceps purpurea: Extension of the cluster sequence and intra species evolution

Thomas Haarmann, Caroline Machado, Yvonne Lübbe, Telmo Correia, Christopher L. Schardl, Daniel G. Panaccione, Paul Tudzynski

Plant Pathology

Research output: Contribution to journal › Article › peer-review

101 Scopus citations

Abstract

The genomic region of Claviceps purpurea strain P1 containing the ergot alkaloid gene cluster [Tudzynski, P., Hölter, K., Correia, T., Arntz, C., Grammel, N., Keller, U., 1999. Evidence for an ergot alkaloid gene cluster in Claviceps purpurea. Mol. Gen. Genet. 261, 133-141] was explored by chromosome walking, and additional genes probably involved in the ergot alkaloid biosynthesis have been identified. The putative cluster sequence (extending over 68.5 kb) contains 4 different nonribosomal peptide synthetase (NRPS) genes and several putative oxidases. Northern analysis showed that most of the genes were co-regulated (repressed by high phosphate), and identified probable flanking genes by lack of co-regulation. Comparison of the cluster sequences of strain P1, an ergotamine producer, with that of strain ECC93, an ergocristine producer, showed high conservation of most of the cluster genes, but significant variation in the NRPS modules, strongly suggesting that evolution of these chemical races of C. purpurea is determined by evolution of NRPS module specificity.

Original language	English
Pages (from-to)	1312-1320
Number of pages	9
Journal	Phytochemistry
Volume	66
Issue number	11 SPEC. ISS.
DOIs	https://doi.org/10.1016/j.phytochem.2005.04.011
State	Published - Jun 2005

Bibliographical note

Funding Information:
This work was supported by the Deutsche Forschungsgemeinschaft (Tu 50/13, SPP 1152: Evolution of metabolic diversity, to P.T.) and the United States Department of Agriculture (2001-35319-10930 and 58-6401-2-0025 to D.G.P. and C.L.S.). We thank N. Lorenz for discussion and for support in the northern analyses, and U. Keller (Berlin) for providing us with unpublished data.

Funding

This work was supported by the Deutsche Forschungsgemeinschaft (Tu 50/13, SPP 1152: Evolution of metabolic diversity, to P.T.) and the United States Department of Agriculture (2001-35319-10930 and 58-6401-2-0025 to D.G.P. and C.L.S.). We thank N. Lorenz for discussion and for support in the northern analyses, and U. Keller (Berlin) for providing us with unpublished data.

Funders	Funder number
C.L.S.
U.S. Department of Agriculture	58-6401-2-0025, 2001-35319-10930
Deutsche Forschungsgemeinschaft	Tu 50/13
Sociedade Portuguesa de Pediatria

Keywords

Chemical races
Claviceps purpurea
Ergocristine
Ergot alkaloids
Ergot fungus
Ergotamine
Gene cluster
Nonribosomal peptide synthetases
Phosphate effect

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Plant Science
Horticulture

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10.1016/j.phytochem.2005.04.011

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@article{db95b0b5a1524478a688aee05500de1a,

title = "The ergot alkaloid gene cluster in Claviceps purpurea: Extension of the cluster sequence and intra species evolution",

abstract = "The genomic region of Claviceps purpurea strain P1 containing the ergot alkaloid gene cluster [Tudzynski, P., H{\"o}lter, K., Correia, T., Arntz, C., Grammel, N., Keller, U., 1999. Evidence for an ergot alkaloid gene cluster in Claviceps purpurea. Mol. Gen. Genet. 261, 133-141] was explored by chromosome walking, and additional genes probably involved in the ergot alkaloid biosynthesis have been identified. The putative cluster sequence (extending over 68.5 kb) contains 4 different nonribosomal peptide synthetase (NRPS) genes and several putative oxidases. Northern analysis showed that most of the genes were co-regulated (repressed by high phosphate), and identified probable flanking genes by lack of co-regulation. Comparison of the cluster sequences of strain P1, an ergotamine producer, with that of strain ECC93, an ergocristine producer, showed high conservation of most of the cluster genes, but significant variation in the NRPS modules, strongly suggesting that evolution of these chemical races of C. purpurea is determined by evolution of NRPS module specificity.",

keywords = "Chemical races, Claviceps purpurea, Ergocristine, Ergot alkaloids, Ergot fungus, Ergotamine, Gene cluster, Nonribosomal peptide synthetases, Phosphate effect",

author = "Thomas Haarmann and Caroline Machado and Yvonne L{\"u}bbe and Telmo Correia and Schardl, {Christopher L.} and Panaccione, {Daniel G.} and Paul Tudzynski",

note = "Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft (Tu 50/13, SPP 1152: Evolution of metabolic diversity, to P.T.) and the United States Department of Agriculture (2001-35319-10930 and 58-6401-2-0025 to D.G.P. and C.L.S.). We thank N. Lorenz for discussion and for support in the northern analyses, and U. Keller (Berlin) for providing us with unpublished data. ",

year = "2005",

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doi = "10.1016/j.phytochem.2005.04.011",

language = "English",

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AU - Haarmann, Thomas

AU - Machado, Caroline

AU - Lübbe, Yvonne

AU - Correia, Telmo

AU - Schardl, Christopher L.

AU - Panaccione, Daniel G.

AU - Tudzynski, Paul

N1 - Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft (Tu 50/13, SPP 1152: Evolution of metabolic diversity, to P.T.) and the United States Department of Agriculture (2001-35319-10930 and 58-6401-2-0025 to D.G.P. and C.L.S.). We thank N. Lorenz for discussion and for support in the northern analyses, and U. Keller (Berlin) for providing us with unpublished data.

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N2 - The genomic region of Claviceps purpurea strain P1 containing the ergot alkaloid gene cluster [Tudzynski, P., Hölter, K., Correia, T., Arntz, C., Grammel, N., Keller, U., 1999. Evidence for an ergot alkaloid gene cluster in Claviceps purpurea. Mol. Gen. Genet. 261, 133-141] was explored by chromosome walking, and additional genes probably involved in the ergot alkaloid biosynthesis have been identified. The putative cluster sequence (extending over 68.5 kb) contains 4 different nonribosomal peptide synthetase (NRPS) genes and several putative oxidases. Northern analysis showed that most of the genes were co-regulated (repressed by high phosphate), and identified probable flanking genes by lack of co-regulation. Comparison of the cluster sequences of strain P1, an ergotamine producer, with that of strain ECC93, an ergocristine producer, showed high conservation of most of the cluster genes, but significant variation in the NRPS modules, strongly suggesting that evolution of these chemical races of C. purpurea is determined by evolution of NRPS module specificity.

AB - The genomic region of Claviceps purpurea strain P1 containing the ergot alkaloid gene cluster [Tudzynski, P., Hölter, K., Correia, T., Arntz, C., Grammel, N., Keller, U., 1999. Evidence for an ergot alkaloid gene cluster in Claviceps purpurea. Mol. Gen. Genet. 261, 133-141] was explored by chromosome walking, and additional genes probably involved in the ergot alkaloid biosynthesis have been identified. The putative cluster sequence (extending over 68.5 kb) contains 4 different nonribosomal peptide synthetase (NRPS) genes and several putative oxidases. Northern analysis showed that most of the genes were co-regulated (repressed by high phosphate), and identified probable flanking genes by lack of co-regulation. Comparison of the cluster sequences of strain P1, an ergotamine producer, with that of strain ECC93, an ergocristine producer, showed high conservation of most of the cluster genes, but significant variation in the NRPS modules, strongly suggesting that evolution of these chemical races of C. purpurea is determined by evolution of NRPS module specificity.

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KW - Phosphate effect

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JO - Phytochemistry

JF - Phytochemistry

IS - 11 SPEC. ISS.

ER -

The ergot alkaloid gene cluster in Claviceps purpurea: Extension of the cluster sequence and intra species evolution

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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