The yellow fever mosquito, Aedes aegypti, affects the health of millions of people by transmitting arboviruses that cause multiple diseases. Since mosquitoes can only transmit viruses in the adult stage through bites, preventing adult emergence by derailing larval development is a promising approach for preventing the transmission of pathogens that cause diseases. Here, we investigate the function of a mitochondrial regulator LRPPRC (leucine‐rich pentatricopeptide repeat containing) in Ae. aegypti and evaluated its potential for mosquito control. The CRISPR/Cas9 genome editing-induced knockout of LRPPRC caused a decrease in larval body size, extended the developmental time of larvae, and increased mortality during the larval and pupal stages. Further studies revealed that LRPPRC is critical to maintaining mitochondrial mRNA poly(A) length and abundance. Behavior and RT-qPCR assays revealed that mitochondrial dysfunction does not affect foraging behavior but disturbs energy hemostasis. Using nanoformulated dsRNA, we show the potential of targeting genes coding for mitochondrial proteins for disease vector control. These findings help advance the understanding of the regulation of mitochondrial function in Ae. aegypti and identify new target genes for mosquito control.
|Journal||Journal of Pest Science|
|State||Accepted/In press - 2023|
Bibliographical noteFunding Information:
Research reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R01GM070559 and the National Institute of Food and Agriculture, US Department of Agriculture (under HATCH Project 2351177000).
We are grateful to Dr. Anil Kumar Moola for his assistance with nanoparticle preparation. Research reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R01GM070559 and the National Institute of Food and Agriculture, US Department of Agriculture (under HATCH Project 2351177000). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
© 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
- dsRNA pesticide
- Insect foraging
- PPR domain
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Agronomy and Crop Science
- Plant Science
- Insect Science