The function of mitochondrial regulator LRPPRC in Aedes aegypti and its potential for mosquito control

Yaoyu Jiao; Guan Heng Zhu; Xien Chen; Ramesh Kumar Dhandapani; Subba Reddy Palli

doi:10.1007/s10340-023-01683-9

The function of mitochondrial regulator LRPPRC in Aedes aegypti and its potential for mosquito control

Yaoyu Jiao, Guan Heng Zhu, Xien Chen, Ramesh Kumar Dhandapani, Subba Reddy Palli

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The yellow fever mosquito, Aedes aegypti, affects the health of millions of people by transmitting arboviruses that cause multiple diseases. Since mosquitoes can only transmit viruses in the adult stage through bites, preventing adult emergence by derailing larval development is a promising approach for preventing the transmission of pathogens that cause diseases. Here, we investigate the function of a mitochondrial regulator LRPPRC (leucine‐rich pentatricopeptide repeat containing) in Ae. aegypti and evaluated its potential for mosquito control. The CRISPR/Cas9 genome editing-induced knockout of LRPPRC caused a decrease in larval body size, extended the developmental time of larvae, and increased mortality during the larval and pupal stages. Further studies revealed that LRPPRC is critical to maintaining mitochondrial mRNA poly(A) length and abundance. Behavior and RT-qPCR assays revealed that mitochondrial dysfunction does not affect foraging behavior but disturbs energy hemostasis. Using nanoformulated dsRNA, we show the potential of targeting genes coding for mitochondrial proteins for disease vector control. These findings help advance the understanding of the regulation of mitochondrial function in Ae. aegypti and identify new target genes for mosquito control.

Original language	English
Pages (from-to)	1045-1057
Number of pages	13
Journal	Journal of Pest Science
Volume	97
Issue number	2
DOIs	https://doi.org/10.1007/s10340-023-01683-9
State	Published - Mar 2024

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023.

Funding

Research reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R01GM070559 and the National Institute of Food and Agriculture, US Department of Agriculture (under HATCH Project 2351177000). We are grateful to Dr. Anil Kumar Moola for his assistance with nanoparticle preparation. Research reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R01GM070559 and the National Institute of Food and Agriculture, US Department of Agriculture (under HATCH Project 2351177000). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Funders	Funder number
National Institutes of Health (NIH)	R01GM070559
National Institute of General Medical Sciences
U.S. Department of Agriculture	2351177000
National Institute of Food and Agriculture

Keywords

Insect foraging
PPR domain
Polyadenylation
dsRNA pesticide

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Ecology
Agronomy and Crop Science
Plant Science
Insect Science

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title = "The function of mitochondrial regulator LRPPRC in Aedes aegypti and its potential for mosquito control",

abstract = "The yellow fever mosquito, Aedes aegypti, affects the health of millions of people by transmitting arboviruses that cause multiple diseases. Since mosquitoes can only transmit viruses in the adult stage through bites, preventing adult emergence by derailing larval development is a promising approach for preventing the transmission of pathogens that cause diseases. Here, we investigate the function of a mitochondrial regulator LRPPRC (leucine‐rich pentatricopeptide repeat containing) in Ae. aegypti and evaluated its potential for mosquito control. The CRISPR/Cas9 genome editing-induced knockout of LRPPRC caused a decrease in larval body size, extended the developmental time of larvae, and increased mortality during the larval and pupal stages. Further studies revealed that LRPPRC is critical to maintaining mitochondrial mRNA poly(A) length and abundance. Behavior and RT-qPCR assays revealed that mitochondrial dysfunction does not affect foraging behavior but disturbs energy hemostasis. Using nanoformulated dsRNA, we show the potential of targeting genes coding for mitochondrial proteins for disease vector control. These findings help advance the understanding of the regulation of mitochondrial function in Ae. aegypti and identify new target genes for mosquito control.",

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note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023.",

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AU - Palli, Subba Reddy

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The function of mitochondrial regulator LRPPRC in Aedes aegypti and its potential for mosquito control

Abstract

Bibliographical note

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Keywords

ASJC Scopus subject areas

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