TY - JOUR
T1 - The generation of human lymphokine-activated killer cells in various serum-free media
AU - Jadus, Martin R.
AU - Thurman, Gary B.
AU - Mrowca-Bastin, Ann
AU - Yannelli, John R.
PY - 1988/5/9
Y1 - 1988/5/9
N2 - Recent data generated in our laboratory indicates that human lymphokine-activated killer (LAK) cells can be induced in several commercially available serum-free medium formulations. Interestingly, LAK cells were also generated in RPMI 1640 medium plus 1000 U/ml IL-2 without addition of human serum. However, higher levels of cytolytic activity were generally obtained in the commercial serum-free formulations. Cytotoxic activity was observed both at 3 and 7 days against a LAK-sensitive cell line (Daudi). Maximal cytolytic activity was usually produced by 7 days of in vitro culture in 1000 U/ml of interleukin-2 (IL-2).
AB - Recent data generated in our laboratory indicates that human lymphokine-activated killer (LAK) cells can be induced in several commercially available serum-free medium formulations. Interestingly, LAK cells were also generated in RPMI 1640 medium plus 1000 U/ml IL-2 without addition of human serum. However, higher levels of cytolytic activity were generally obtained in the commercial serum-free formulations. Cytotoxic activity was observed both at 3 and 7 days against a LAK-sensitive cell line (Daudi). Maximal cytolytic activity was usually produced by 7 days of in vitro culture in 1000 U/ml of interleukin-2 (IL-2).
KW - Interleukin-2
KW - Lymphokine-activated killer cell
KW - Serum-free medium
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U2 - 10.1016/0022-1759(88)90239-6
DO - 10.1016/0022-1759(88)90239-6
M3 - Article
C2 - 3361130
AN - SCOPUS:0023911868
SN - 0022-1759
VL - 109
SP - 169
EP - 174
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 2
ER -