The generation of human lymphokine-activated killer cells in various serum-free media

Martin R. Jadus; Gary B. Thurman; Ann Mrowca-Bastin; John R. Yannelli

doi:10.1016/0022-1759(88)90239-6

The generation of human lymphokine-activated killer cells in various serum-free media

Martin R. Jadus, Gary B. Thurman, Ann Mrowca-Bastin, John R. Yannelli

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Recent data generated in our laboratory indicates that human lymphokine-activated killer (LAK) cells can be induced in several commercially available serum-free medium formulations. Interestingly, LAK cells were also generated in RPMI 1640 medium plus 1000 U/ml IL-2 without addition of human serum. However, higher levels of cytolytic activity were generally obtained in the commercial serum-free formulations. Cytotoxic activity was observed both at 3 and 7 days against a LAK-sensitive cell line (Daudi). Maximal cytolytic activity was usually produced by 7 days of in vitro culture in 1000 U/ml of interleukin-2 (IL-2).

Original language	English
Pages (from-to)	169-174
Number of pages	6
Journal	Journal of Immunological Methods
Volume	109
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(88)90239-6
State	Published - May 9 1988

Keywords

Interleukin-2
Lymphokine-activated killer cell
Serum-free medium

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(88)90239-6

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title = "The generation of human lymphokine-activated killer cells in various serum-free media",

abstract = "Recent data generated in our laboratory indicates that human lymphokine-activated killer (LAK) cells can be induced in several commercially available serum-free medium formulations. Interestingly, LAK cells were also generated in RPMI 1640 medium plus 1000 U/ml IL-2 without addition of human serum. However, higher levels of cytolytic activity were generally obtained in the commercial serum-free formulations. Cytotoxic activity was observed both at 3 and 7 days against a LAK-sensitive cell line (Daudi). Maximal cytolytic activity was usually produced by 7 days of in vitro culture in 1000 U/ml of interleukin-2 (IL-2).",

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AU - Thurman, Gary B.

AU - Mrowca-Bastin, Ann

AU - Yannelli, John R.

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AB - Recent data generated in our laboratory indicates that human lymphokine-activated killer (LAK) cells can be induced in several commercially available serum-free medium formulations. Interestingly, LAK cells were also generated in RPMI 1640 medium plus 1000 U/ml IL-2 without addition of human serum. However, higher levels of cytolytic activity were generally obtained in the commercial serum-free formulations. Cytotoxic activity was observed both at 3 and 7 days against a LAK-sensitive cell line (Daudi). Maximal cytolytic activity was usually produced by 7 days of in vitro culture in 1000 U/ml of interleukin-2 (IL-2).

KW - Interleukin-2

KW - Lymphokine-activated killer cell

KW - Serum-free medium

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The generation of human lymphokine-activated killer cells in various serum-free media

Abstract

Keywords

ASJC Scopus subject areas

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