The generation of human lymphokine-activated killer cells in various serum-free media

Martin R. Jadus, Gary B. Thurman, Ann Mrowca-Bastin, John R. Yannelli

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Recent data generated in our laboratory indicates that human lymphokine-activated killer (LAK) cells can be induced in several commercially available serum-free medium formulations. Interestingly, LAK cells were also generated in RPMI 1640 medium plus 1000 U/ml IL-2 without addition of human serum. However, higher levels of cytolytic activity were generally obtained in the commercial serum-free formulations. Cytotoxic activity was observed both at 3 and 7 days against a LAK-sensitive cell line (Daudi). Maximal cytolytic activity was usually produced by 7 days of in vitro culture in 1000 U/ml of interleukin-2 (IL-2).

Original languageEnglish
Pages (from-to)169-174
Number of pages6
JournalJournal of Immunological Methods
Volume109
Issue number2
DOIs
StatePublished - May 9 1988

Keywords

  • Interleukin-2
  • Lymphokine-activated killer cell
  • Serum-free medium

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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