Abstract
Recent data generated in our laboratory indicates that human lymphokine-activated killer (LAK) cells can be induced in several commercially available serum-free medium formulations. Interestingly, LAK cells were also generated in RPMI 1640 medium plus 1000 U/ml IL-2 without addition of human serum. However, higher levels of cytolytic activity were generally obtained in the commercial serum-free formulations. Cytotoxic activity was observed both at 3 and 7 days against a LAK-sensitive cell line (Daudi). Maximal cytolytic activity was usually produced by 7 days of in vitro culture in 1000 U/ml of interleukin-2 (IL-2).
| Original language | English |
|---|---|
| Pages (from-to) | 169-174 |
| Number of pages | 6 |
| Journal | Journal of Immunological Methods |
| Volume | 109 |
| Issue number | 2 |
| DOIs | |
| State | Published - May 9 1988 |
Keywords
- Interleukin-2
- Lymphokine-activated killer cell
- Serum-free medium
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
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