The genome sequence of the rice blast fungus Magnaporthe grisea

Ralph A. Dean; Nicholas J. Talbot; Daniel J. Ebbole; Mark L. Farman; Thomas K. Mitchell; Marc J. Orbach; Michael Thon; Resham Kulkarni; Jin Rong Xu; Huaqin Pan; Nick D. Read; Yong Ilwan Lee; Ignazio Carbone; Doug Brown; Yee Oh Yeon; Nicole Donofrio; Seop Jeong Jun; Darren M. Soanes; Slavica Djonovic; Elena Kolomlots; Cathryn Rehmeyer; Weixl Li; Michael Harding; Soonok Kim; Marc Henri Lebrun; Heidi Bohnert; Sean Coughlan; Jonathan Butler; Sarah Calvo; Li Jun Ma; Robert Nicol; Seth Purcell; Chad Nusbaum; James E. Galagan; Bruce W. Dirren

doi:10.1038/nature03449

The genome sequence of the rice blast fungus Magnaporthe grisea

Ralph A. Dean, Nicholas J. Talbot, Daniel J. Ebbole, Mark L. Farman, Thomas K. Mitchell, Marc J. Orbach, Michael Thon, Resham Kulkarni, Jin Rong Xu, Huaqin Pan, Nick D. Read, Yong Ilwan Lee, Ignazio Carbone, Doug Brown, Yee Oh Yeon, Nicole Donofrio, Seop Jeong Jun, Darren M. Soanes, Slavica Djonovic, Elena KolomlotsCathryn Rehmeyer, Weixl Li, Michael Harding, Soonok Kim, Marc Henri Lebrun, Heidi Bohnert, Sean Coughlan, Jonathan Butler, Sarah Calvo, Li Jun Ma, Robert Nicol, Seth Purcell, Chad Nusbaum, James E. Galagan, Bruce W. Dirren

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Abstract

Magnaporthe grisea is the most destructive pathogen of rice worldwide and the principal model organism for elucidating the molecular basis of fungal disease of plants. Here, we report the draft sequence of the M. grisea genome. Analysis of the gene set provides an insight into the adaptations required by a fungus to cause disease. The genome encodes a large and diverse set of secreted proteins, including those defined by unusual carbohydrate-binding domains. This fungus also possesses an expanded family of G-protein-coupled receptors, several new virulence-associated genes and large suites of enzymes involved in secondary metabolism. Consistent with a role in fungal pathogenesis, the expression of several of these genes is upregulated during the early stages of infection-related development. The M. grisea genome has been subject to invasion and proliferation of active transposable elements, reflecting the clonal nature of this fungus imposed by widespread rice cultivation.

Original language	English
Pages (from-to)	980-986
Number of pages	7
Journal	Nature
Volume	434
Issue number	7036
DOIs	https://doi.org/10.1038/nature03449
State	Published - Apr 21 2005

Bibliographical note

Funding Information:
Acknowledgements The authors acknowledge the USDA-CSREES and the National Science Foundation for funding this work. We thank all the members of the Dean Laboratory at NCSU, and members of the Broad Institute Sequencing Platform, Assembly and Annotation teams, and members at each collaborating laboratory. We also thank the rice blast research community at large. We acknowledge other fungal research communities, in particular the A. nidulans community, for making it possible to have access to genome sequence information before publication.

Funding

Acknowledgements The authors acknowledge the USDA-CSREES and the National Science Foundation for funding this work. We thank all the members of the Dean Laboratory at NCSU, and members of the Broad Institute Sequencing Platform, Assembly and Annotation teams, and members at each collaborating laboratory. We also thank the rice blast research community at large. We acknowledge other fungal research communities, in particular the A. nidulans community, for making it possible to have access to genome sequence information before publication.

Funders	Funder number
USDA-CSREES
National Science Foundation Arctic Social Science Program

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Dean, R. A., Talbot, N. J., Ebbole, D. J., Farman, M. L., Mitchell, T. K., Orbach, M. J., Thon, M., Kulkarni, R., Xu, J. R., Pan, H., Read, N. D., Lee, Y. I., Carbone, I., Brown, D., Yeon, Y. O., Donofrio, N., Jun, S. J., Soanes, D. M., Djonovic, S., ... Dirren, B. W. (2005). The genome sequence of the rice blast fungus Magnaporthe grisea. Nature, 434(7036), 980-986. https://doi.org/10.1038/nature03449

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title = "The genome sequence of the rice blast fungus Magnaporthe grisea",

abstract = "Magnaporthe grisea is the most destructive pathogen of rice worldwide and the principal model organism for elucidating the molecular basis of fungal disease of plants. Here, we report the draft sequence of the M. grisea genome. Analysis of the gene set provides an insight into the adaptations required by a fungus to cause disease. The genome encodes a large and diverse set of secreted proteins, including those defined by unusual carbohydrate-binding domains. This fungus also possesses an expanded family of G-protein-coupled receptors, several new virulence-associated genes and large suites of enzymes involved in secondary metabolism. Consistent with a role in fungal pathogenesis, the expression of several of these genes is upregulated during the early stages of infection-related development. The M. grisea genome has been subject to invasion and proliferation of active transposable elements, reflecting the clonal nature of this fungus imposed by widespread rice cultivation.",

author = "Dean, {Ralph A.} and Talbot, {Nicholas J.} and Ebbole, {Daniel J.} and Farman, {Mark L.} and Mitchell, {Thomas K.} and Orbach, {Marc J.} and Michael Thon and Resham Kulkarni and Xu, {Jin Rong} and Huaqin Pan and Read, {Nick D.} and Lee, {Yong Ilwan} and Ignazio Carbone and Doug Brown and Yeon, {Yee Oh} and Nicole Donofrio and Jun, {Seop Jeong} and Soanes, {Darren M.} and Slavica Djonovic and Elena Kolomlots and Cathryn Rehmeyer and Weixl Li and Michael Harding and Soonok Kim and Lebrun, {Marc Henri} and Heidi Bohnert and Sean Coughlan and Jonathan Butler and Sarah Calvo and Ma, {Li Jun} and Robert Nicol and Seth Purcell and Chad Nusbaum and Galagan, {James E.} and Dirren, {Bruce W.}",

note = "Funding Information: Acknowledgements The authors acknowledge the USDA-CSREES and the National Science Foundation for funding this work. We thank all the members of the Dean Laboratory at NCSU, and members of the Broad Institute Sequencing Platform, Assembly and Annotation teams, and members at each collaborating laboratory. We also thank the rice blast research community at large. We acknowledge other fungal research communities, in particular the A. nidulans community, for making it possible to have access to genome sequence information before publication.",

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Dean, RA, Talbot, NJ, Ebbole, DJ, Farman, ML, Mitchell, TK, Orbach, MJ, Thon, M, Kulkarni, R, Xu, JR, Pan, H, Read, ND, Lee, YI, Carbone, I, Brown, D, Yeon, YO, Donofrio, N, Jun, SJ, Soanes, DM, Djonovic, S, Kolomlots, E, Rehmeyer, C, Li, W, Harding, M, Kim, S, Lebrun, MH, Bohnert, H, Coughlan, S, Butler, J, Calvo, S, Ma, LJ, Nicol, R, Purcell, S, Nusbaum, C, Galagan, JE & Dirren, BW 2005, 'The genome sequence of the rice blast fungus Magnaporthe grisea', Nature, vol. 434, no. 7036, pp. 980-986. https://doi.org/10.1038/nature03449

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T1 - The genome sequence of the rice blast fungus Magnaporthe grisea

AU - Dean, Ralph A.

AU - Talbot, Nicholas J.

AU - Ebbole, Daniel J.

AU - Farman, Mark L.

AU - Mitchell, Thomas K.

AU - Orbach, Marc J.

AU - Thon, Michael

AU - Kulkarni, Resham

AU - Xu, Jin Rong

AU - Pan, Huaqin

AU - Read, Nick D.

AU - Lee, Yong Ilwan

AU - Carbone, Ignazio

AU - Brown, Doug

AU - Yeon, Yee Oh

AU - Donofrio, Nicole

AU - Jun, Seop Jeong

AU - Soanes, Darren M.

AU - Djonovic, Slavica

AU - Kolomlots, Elena

AU - Rehmeyer, Cathryn

AU - Li, Weixl

AU - Harding, Michael

AU - Kim, Soonok

AU - Lebrun, Marc Henri

AU - Bohnert, Heidi

AU - Coughlan, Sean

AU - Butler, Jonathan

AU - Calvo, Sarah

AU - Ma, Li Jun

AU - Nicol, Robert

AU - Purcell, Seth

AU - Nusbaum, Chad

AU - Galagan, James E.

AU - Dirren, Bruce W.

N1 - Funding Information: Acknowledgements The authors acknowledge the USDA-CSREES and the National Science Foundation for funding this work. We thank all the members of the Dean Laboratory at NCSU, and members of the Broad Institute Sequencing Platform, Assembly and Annotation teams, and members at each collaborating laboratory. We also thank the rice blast research community at large. We acknowledge other fungal research communities, in particular the A. nidulans community, for making it possible to have access to genome sequence information before publication.

PY - 2005/4/21

Y1 - 2005/4/21

N2 - Magnaporthe grisea is the most destructive pathogen of rice worldwide and the principal model organism for elucidating the molecular basis of fungal disease of plants. Here, we report the draft sequence of the M. grisea genome. Analysis of the gene set provides an insight into the adaptations required by a fungus to cause disease. The genome encodes a large and diverse set of secreted proteins, including those defined by unusual carbohydrate-binding domains. This fungus also possesses an expanded family of G-protein-coupled receptors, several new virulence-associated genes and large suites of enzymes involved in secondary metabolism. Consistent with a role in fungal pathogenesis, the expression of several of these genes is upregulated during the early stages of infection-related development. The M. grisea genome has been subject to invasion and proliferation of active transposable elements, reflecting the clonal nature of this fungus imposed by widespread rice cultivation.

AB - Magnaporthe grisea is the most destructive pathogen of rice worldwide and the principal model organism for elucidating the molecular basis of fungal disease of plants. Here, we report the draft sequence of the M. grisea genome. Analysis of the gene set provides an insight into the adaptations required by a fungus to cause disease. The genome encodes a large and diverse set of secreted proteins, including those defined by unusual carbohydrate-binding domains. This fungus also possesses an expanded family of G-protein-coupled receptors, several new virulence-associated genes and large suites of enzymes involved in secondary metabolism. Consistent with a role in fungal pathogenesis, the expression of several of these genes is upregulated during the early stages of infection-related development. The M. grisea genome has been subject to invasion and proliferation of active transposable elements, reflecting the clonal nature of this fungus imposed by widespread rice cultivation.

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The genome sequence of the rice blast fungus Magnaporthe grisea

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