TY - JOUR
T1 - The giant axolotl genome uncovers the evolution, scaling, and transcriptional control of complex gene loci
AU - Schloissnig, Siegfried
AU - Kawaguchi, Akane
AU - Nowoshilow, Sergej
AU - Falcon, Francisco
AU - Otsuki, Leo
AU - Tardivo, Pietro
AU - Timoshevskaya, Nataliya
AU - Keinath, Melissa C.
AU - Smith, Jeramiah James
AU - Voss, S. Randal
AU - Tanaka, Elly M.
N1 - Publisher Copyright:
© This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
PY - 2021/4/13
Y1 - 2021/4/13
N2 - Vertebrates harbor recognizably orthologous gene complements but vary 100-fold in genome size. How chromosomal organization scales with genome expansion is unclear, and how acute changes in gene regulation, as during axolotl limb regeneration, occur in the context of a vast genome has remained a riddle. Here, we describe the chromosome-scale assembly of the giant, 32 Gb axolotl genome. Hi-C contact data revealed the scaling properties of interphase and mitotic chromosome organization. Analysis of the assembly yielded understanding of the evolution of large, syntenic multigene clusters, including the Major Histocompatibility Complex (MHC) and the functional regulatory landscape of the Fibroblast Growth Factor 8 (Axfgf8) region. The axolotl serves as a primary model for studying successful regeneration.
AB - Vertebrates harbor recognizably orthologous gene complements but vary 100-fold in genome size. How chromosomal organization scales with genome expansion is unclear, and how acute changes in gene regulation, as during axolotl limb regeneration, occur in the context of a vast genome has remained a riddle. Here, we describe the chromosome-scale assembly of the giant, 32 Gb axolotl genome. Hi-C contact data revealed the scaling properties of interphase and mitotic chromosome organization. Analysis of the assembly yielded understanding of the evolution of large, syntenic multigene clusters, including the Major Histocompatibility Complex (MHC) and the functional regulatory landscape of the Fibroblast Growth Factor 8 (Axfgf8) region. The axolotl serves as a primary model for studying successful regeneration.
KW - Axolotl
KW - Genome assembly
KW - Regeneration
KW - Topological associating domains
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U2 - 10.1073/pnas.2017176118
DO - 10.1073/pnas.2017176118
M3 - Article
C2 - 33827918
AN - SCOPUS:85103998319
SN - 0027-8424
VL - 118
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 15
M1 - e2017176118
ER -