The histone variant H2A.W defines heterochromatin and promotes chromatin condensation in arabidopsis

Ramesh Yelagandula; Hume Stroud; Sarah Holec; Keda Zhou; Suhua Feng; Xuehua Zhong; Uma M. Muthurajan; Xin Nie; Tomokazu Kawashima; Martin Groth; Karolin Luger; Steven E. Jacobsen; Frédéric Berger

doi:10.1016/j.cell.2014.06.006

The histone variant H2A.W defines heterochromatin and promotes chromatin condensation in arabidopsis

Ramesh Yelagandula, Hume Stroud, Sarah Holec, Keda Zhou, Suhua Feng, Xuehua Zhong, Uma M. Muthurajan, Xin Nie, Tomokazu Kawashima, Martin Groth, Karolin Luger, Steven E. Jacobsen, Frédéric Berger

Research output: Contribution to journal › Article › peer-review

210 Scopus citations

Abstract

Histone variants play crucial roles in gene expression, genome integrity, and chromosome segregation. We report that the four H2A variants in Arabidopsis define different genomic features, contributing to overall genomic organization. The histone variant H2A.W marks heterochromatin specifically and acts in synergy with heterochromatic marks H3K9me2 and DNA methylation to maintain transposon silencing. In vitro, H2A.W enhances chromatin condensation by promoting fiber-to-fiber interactions via its conserved C-terminal motif. In vivo, H2A.W is required for heterochromatin condensation, demonstrating that H2A.W plays critical roles in heterochromatin organization. Similarities in conserved motifs between H2A.W and another H2A variant in metazoans suggest that plants and animals share common mechanisms for heterochromatin condensation.

Original language	English
Pages (from-to)	98-109
Number of pages	12
Journal	Cell
Volume	158
Issue number	1
DOIs	https://doi.org/10.1016/j.cell.2014.06.006
State	Published - Jul 3 2014

Bibliographical note

Funding Information:
R.Y., S.H., X.N., and F.B. were funded by Temasek Life Sciences Laboratory. Sequencing was performed at the UCLA BSCRC BioSequencing Core facility. We thank Meredith Calvert for help with imaging and Mahnaz Akhavan for Illumina sequencing. We thank Dr. Maruyama for his comments and support with the graphical abstract. H.S. was supported by a Dissertation Year Fellowship from UCLA. X.Z. is a research fellow of Ruth L. Kirschstein National Research Service Award (F32GM096483-01). S.F. is a Special Fellow of the Leukemia & Lymphoma Society. Work in the Jacobsen lab was supported by NSF grant 1121245. K.L., U.M., and K.Z. are supported by NIH-GM067777. M.G. is supported by EMBO ALTF 986-2011 and HHMI. S.E.J. and K.L. are Investigators of the Howard Hughes Medical Institute.

Funding

R.Y., S.H., X.N., and F.B. were funded by Temasek Life Sciences Laboratory. Sequencing was performed at the UCLA BSCRC BioSequencing Core facility. We thank Meredith Calvert for help with imaging and Mahnaz Akhavan for Illumina sequencing. We thank Dr. Maruyama for his comments and support with the graphical abstract. H.S. was supported by a Dissertation Year Fellowship from UCLA. X.Z. is a research fellow of Ruth L. Kirschstein National Research Service Award (F32GM096483-01). S.F. is a Special Fellow of the Leukemia & Lymphoma Society. Work in the Jacobsen lab was supported by NSF grant 1121245. K.L., U.M., and K.Z. are supported by NIH-GM067777. M.G. is supported by EMBO ALTF 986-2011 and HHMI. S.E.J. and K.L. are Investigators of the Howard Hughes Medical Institute.

Funders	Funder number
National Science Foundation (NSF)	1121245
Howard Hughes Medical Institute
National Institute of General Medical Sciences	R01GM067777
European Molecular Biology Organization	ALTF 986-2011
University of California, Los Angeles
Temasek Life Sciences Laboratory

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.cell.2014.06.006

Cite this

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title = "The histone variant H2A.W defines heterochromatin and promotes chromatin condensation in arabidopsis",

abstract = "Histone variants play crucial roles in gene expression, genome integrity, and chromosome segregation. We report that the four H2A variants in Arabidopsis define different genomic features, contributing to overall genomic organization. The histone variant H2A.W marks heterochromatin specifically and acts in synergy with heterochromatic marks H3K9me2 and DNA methylation to maintain transposon silencing. In vitro, H2A.W enhances chromatin condensation by promoting fiber-to-fiber interactions via its conserved C-terminal motif. In vivo, H2A.W is required for heterochromatin condensation, demonstrating that H2A.W plays critical roles in heterochromatin organization. Similarities in conserved motifs between H2A.W and another H2A variant in metazoans suggest that plants and animals share common mechanisms for heterochromatin condensation.",

author = "Ramesh Yelagandula and Hume Stroud and Sarah Holec and Keda Zhou and Suhua Feng and Xuehua Zhong and Muthurajan, {Uma M.} and Xin Nie and Tomokazu Kawashima and Martin Groth and Karolin Luger and Jacobsen, {Steven E.} and Fr{\'e}d{\'e}ric Berger",

note = "Funding Information: R.Y., S.H., X.N., and F.B. were funded by Temasek Life Sciences Laboratory. Sequencing was performed at the UCLA BSCRC BioSequencing Core facility. We thank Meredith Calvert for help with imaging and Mahnaz Akhavan for Illumina sequencing. We thank Dr. Maruyama for his comments and support with the graphical abstract. H.S. was supported by a Dissertation Year Fellowship from UCLA. X.Z. is a research fellow of Ruth L. Kirschstein National Research Service Award (F32GM096483-01). S.F. is a Special Fellow of the Leukemia & Lymphoma Society. Work in the Jacobsen lab was supported by NSF grant 1121245. K.L., U.M., and K.Z. are supported by NIH-GM067777. M.G. is supported by EMBO ALTF 986-2011 and HHMI. S.E.J. and K.L. are Investigators of the Howard Hughes Medical Institute. ",

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AU - Yelagandula, Ramesh

AU - Stroud, Hume

AU - Holec, Sarah

AU - Zhou, Keda

AU - Feng, Suhua

AU - Zhong, Xuehua

AU - Muthurajan, Uma M.

AU - Nie, Xin

AU - Kawashima, Tomokazu

AU - Groth, Martin

AU - Luger, Karolin

AU - Jacobsen, Steven E.

AU - Berger, Frédéric

N1 - Funding Information: R.Y., S.H., X.N., and F.B. were funded by Temasek Life Sciences Laboratory. Sequencing was performed at the UCLA BSCRC BioSequencing Core facility. We thank Meredith Calvert for help with imaging and Mahnaz Akhavan for Illumina sequencing. We thank Dr. Maruyama for his comments and support with the graphical abstract. H.S. was supported by a Dissertation Year Fellowship from UCLA. X.Z. is a research fellow of Ruth L. Kirschstein National Research Service Award (F32GM096483-01). S.F. is a Special Fellow of the Leukemia & Lymphoma Society. Work in the Jacobsen lab was supported by NSF grant 1121245. K.L., U.M., and K.Z. are supported by NIH-GM067777. M.G. is supported by EMBO ALTF 986-2011 and HHMI. S.E.J. and K.L. are Investigators of the Howard Hughes Medical Institute.

PY - 2014/7/3

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N2 - Histone variants play crucial roles in gene expression, genome integrity, and chromosome segregation. We report that the four H2A variants in Arabidopsis define different genomic features, contributing to overall genomic organization. The histone variant H2A.W marks heterochromatin specifically and acts in synergy with heterochromatic marks H3K9me2 and DNA methylation to maintain transposon silencing. In vitro, H2A.W enhances chromatin condensation by promoting fiber-to-fiber interactions via its conserved C-terminal motif. In vivo, H2A.W is required for heterochromatin condensation, demonstrating that H2A.W plays critical roles in heterochromatin organization. Similarities in conserved motifs between H2A.W and another H2A variant in metazoans suggest that plants and animals share common mechanisms for heterochromatin condensation.

AB - Histone variants play crucial roles in gene expression, genome integrity, and chromosome segregation. We report that the four H2A variants in Arabidopsis define different genomic features, contributing to overall genomic organization. The histone variant H2A.W marks heterochromatin specifically and acts in synergy with heterochromatic marks H3K9me2 and DNA methylation to maintain transposon silencing. In vitro, H2A.W enhances chromatin condensation by promoting fiber-to-fiber interactions via its conserved C-terminal motif. In vivo, H2A.W is required for heterochromatin condensation, demonstrating that H2A.W plays critical roles in heterochromatin organization. Similarities in conserved motifs between H2A.W and another H2A variant in metazoans suggest that plants and animals share common mechanisms for heterochromatin condensation.

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The histone variant H2A.W defines heterochromatin and promotes chromatin condensation in arabidopsis

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

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