The impact of enzyme engineering upon natural product glycodiversification

Gavin J. Williams; Richard W. Gantt; Jon S. Thorson

doi:10.1016/j.cbpa.2008.07.013

The impact of enzyme engineering upon natural product glycodiversification

Gavin J. Williams, Richard W. Gantt, Jon S. Thorson

Research output: Contribution to journal › Review article › peer-review

89 Scopus citations

Abstract

Glycodiversification of natural products is an effective strategy for small molecule drug development. Recently, improved methods for chemo-enzymatic synthesis of glycosyl donors has spurred the characterization of natural product glycosyltransferases (GTs), revealing that the substrate specificity of many naturally occurring GTs as too stringent for use in glycodiversification. Protein engineering of natural product GTs has emerged as an attractive approach to overcome this limitation. This review highlights recent progress in the engineering/evolution of enzymes relevant to natural product glycodiversification with a particular focus upon GTs.

Original language	English
Pages (from-to)	556-564
Number of pages	9
Journal	Current Opinion in Chemical Biology
Volume	12
Issue number	5
DOIs	https://doi.org/10.1016/j.cbpa.2008.07.013
State	Published - Oct 2008

Bibliographical note

Funding Information:
This contribution was supported in part by National Institutes of Health Grants CA84374 and U19 CA113297 (JTS). JST is an H.I. Romnes fellow.

Funding

This contribution was supported in part by National Institutes of Health Grants CA84374 and U19 CA113297 (JTS). JST is an H.I. Romnes fellow.

Funders	Funder number
National Institutes of Health (NIH)	U19 CA113297
National Institutes of Health (NIH)
National Childhood Cancer Registry – National Cancer Institute	R01CA084374
National Childhood Cancer Registry – National Cancer Institute

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

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10.1016/j.cbpa.2008.07.013

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title = "The impact of enzyme engineering upon natural product glycodiversification",

abstract = "Glycodiversification of natural products is an effective strategy for small molecule drug development. Recently, improved methods for chemo-enzymatic synthesis of glycosyl donors has spurred the characterization of natural product glycosyltransferases (GTs), revealing that the substrate specificity of many naturally occurring GTs as too stringent for use in glycodiversification. Protein engineering of natural product GTs has emerged as an attractive approach to overcome this limitation. This review highlights recent progress in the engineering/evolution of enzymes relevant to natural product glycodiversification with a particular focus upon GTs.",

author = "Williams, \{Gavin J.\} and Gantt, \{Richard W.\} and Thorson, \{Jon S.\}",

note = "Funding Information: This contribution was supported in part by National Institutes of Health Grants CA84374 and U19 CA113297 (JTS). JST is an H.I. Romnes fellow. ",

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AU - Gantt, Richard W.

AU - Thorson, Jon S.

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N2 - Glycodiversification of natural products is an effective strategy for small molecule drug development. Recently, improved methods for chemo-enzymatic synthesis of glycosyl donors has spurred the characterization of natural product glycosyltransferases (GTs), revealing that the substrate specificity of many naturally occurring GTs as too stringent for use in glycodiversification. Protein engineering of natural product GTs has emerged as an attractive approach to overcome this limitation. This review highlights recent progress in the engineering/evolution of enzymes relevant to natural product glycodiversification with a particular focus upon GTs.

AB - Glycodiversification of natural products is an effective strategy for small molecule drug development. Recently, improved methods for chemo-enzymatic synthesis of glycosyl donors has spurred the characterization of natural product glycosyltransferases (GTs), revealing that the substrate specificity of many naturally occurring GTs as too stringent for use in glycodiversification. Protein engineering of natural product GTs has emerged as an attractive approach to overcome this limitation. This review highlights recent progress in the engineering/evolution of enzymes relevant to natural product glycodiversification with a particular focus upon GTs.

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JO - Current Opinion in Chemical Biology

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ER -

The impact of enzyme engineering upon natural product glycodiversification

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

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