The in vitro characterization of polyene glycosyltransferases AmphDI and NysDI

Changsheng Zhang, Rocco Moretti, Jiqing Jiang, Jon S. Thorson

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

The overproduction, purification, and in vitro characterization of the polyene glycosyltransferases (GTs) AmphDI and NysDI are reported. A novel nucleotidyltransferase mutant (RmlA Q83D) for the chemoenzymatic synthesis of unnatural GDP-sugar donors in conjunction with polyene GT-catalyzed sugar exchange/reverse reactions allowed the donor and acceptor specificities of these novel enzymes to be probed. The evaluation of polyene GT aglycon and GDP-sugar donor specificity revealed some tolerance to aglycon structural diversity, but stringent sugar specificity, and culminated in new polyene analogues in which L-gulose or D-mannose replace the native sugar D-mycosamine.

Original languageEnglish
Pages (from-to)2506-2514
Number of pages9
JournalChemBioChem
Volume9
Issue number15
DOIs
StatePublished - Oct 13 2008

Keywords

  • Antifungal
  • Carbohydrates
  • Drugs
  • Glycosylation
  • Sugars

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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