The in vitro characterization of the erythronolide mycarosyltransferase EryBV and its utility in macrolide diversification

Changsheng Zhang; Qiang Fu; Christoph Albermann; Lingjun Li; Jon S. Thorson

doi:10.1002/cbic.200600509

The in vitro characterization of the erythronolide mycarosyltransferase EryBV and its utility in macrolide diversification

Changsheng Zhang, Qiang Fu, Christoph Albermann, Lingjun Li, Jon S. Thorson

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

(Chemical Equation Presented) By capitalizing upon the reversibility of glycosyltransferase-catalyzed reactions, EryBV was characterized in vitro as the erythromycin mycarosyltransferase and the EryBV substrate specificity was investigated. Two-GT (EryBV/AveBI) aglycon-exchange reactions also led to the production of novel hybrid "erythromycin-avermectin" macrolides.

Original language	English
Pages (from-to)	385-390
Number of pages	6
Journal	ChemBioChem
Volume	8
Issue number	4
DOIs	https://doi.org/10.1002/cbic.200600509
State	Published - Mar 5 2007

Keywords

Biosynthesis
Carbohydrates
Catalysis
Glycosyltransferases
Natural products

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.200600509

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AU - Zhang, Changsheng

AU - Fu, Qiang

AU - Albermann, Christoph

AU - Li, Lingjun

AU - Thorson, Jon S.

PY - 2007/3/5

Y1 - 2007/3/5

N2 - (Chemical Equation Presented) By capitalizing upon the reversibility of glycosyltransferase-catalyzed reactions, EryBV was characterized in vitro as the erythromycin mycarosyltransferase and the EryBV substrate specificity was investigated. Two-GT (EryBV/AveBI) aglycon-exchange reactions also led to the production of novel hybrid "erythromycin-avermectin" macrolides.

AB - (Chemical Equation Presented) By capitalizing upon the reversibility of glycosyltransferase-catalyzed reactions, EryBV was characterized in vitro as the erythromycin mycarosyltransferase and the EryBV substrate specificity was investigated. Two-GT (EryBV/AveBI) aglycon-exchange reactions also led to the production of novel hybrid "erythromycin-avermectin" macrolides.

KW - Biosynthesis

KW - Carbohydrates

KW - Catalysis

KW - Glycosyltransferases

KW - Natural products

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The in vitro characterization of the erythronolide mycarosyltransferase EryBV and its utility in macrolide diversification

Abstract

Keywords

ASJC Scopus subject areas

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