The in vitro characterization of the erythronolide mycarosyltransferase EryBV and its utility in macrolide diversification

  • Changsheng Zhang
  • , Qiang Fu
  • , Christoph Albermann
  • , Lingjun Li
  • , Jon S. Thorson

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

(Chemical Equation Presented) By capitalizing upon the reversibility of glycosyltransferase-catalyzed reactions, EryBV was characterized in vitro as the erythromycin mycarosyltransferase and the EryBV substrate specificity was investigated. Two-GT (EryBV/AveBI) aglycon-exchange reactions also led to the production of novel hybrid "erythromycin-avermectin" macrolides.

Original languageEnglish
Pages (from-to)385-390
Number of pages6
JournalChemBioChem
Volume8
Issue number4
DOIs
StatePublished - Mar 5 2007

Funding

FundersFunder number
National Childhood Cancer Registry – National Cancer InstituteU19CA113297

    Keywords

    • Biosynthesis
    • Carbohydrates
    • Catalysis
    • Glycosyltransferases
    • Natural products

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Medicine
    • Molecular Biology
    • Organic Chemistry

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