The interaction of divalent metal ions with tris buffer in dilute solution

David P. Hanlon; David S. Watt; Edward W. Westhead

doi:10.1016/0003-2697(66)90150-3

The interaction of divalent metal ions with tris buffer in dilute solution

David P. Hanlon, David S. Watt, Edward W. Westhead

Molecular and Cellular Biochemistry

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Abstract

The interaction of 2-amino-2-(hydroxymethyl)-1,3-propanediol (tris) with eight divalent metal ions has been investigated under conditions commonly employed in biochemical studies. Formation constants have been calculated, where possible from pH titration data. Hg⁺⁺ and Cu⁺⁺ interact strongly with tris, and Co⁺⁺, Zn⁺⁺, Cd⁺⁺ much less so. The interaction of tris with Mg⁺⁺ and Mn⁺⁺ is too weak to be detected under the conditions used. Above pH 7.5 further reaction of the metal complexes with added hydroxyl ion complicates the calculation of formation constants for higher complexes of divalent metal ions and Tris.

Original language	English
Pages (from-to)	225-233
Number of pages	9
Journal	Analytical Biochemistry
Volume	16
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(66)90150-3
State	Published - Aug 1966

Bibliographical note

Funding Information:
1 This investigation was supported in part, by a Public Health service (1-F2-GM-19,227-01Al) to D.P.H. from the National Institute of Arthritis bolic Diseases, and in part by grant, GM OS474 to E.W.W. 2 Imdergraduate Research Assistant, Kational lnstit,utes 225

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "The interaction of divalent metal ions with tris buffer in dilute solution",

abstract = "The interaction of 2-amino-2-(hydroxymethyl)-1,3-propanediol (tris) with eight divalent metal ions has been investigated under conditions commonly employed in biochemical studies. Formation constants have been calculated, where possible from pH titration data. Hg++ and Cu++ interact strongly with tris, and Co++, Zn++, Cd++ much less so. The interaction of tris with Mg++ and Mn++ is too weak to be detected under the conditions used. Above pH 7.5 further reaction of the metal complexes with added hydroxyl ion complicates the calculation of formation constants for higher complexes of divalent metal ions and Tris.",

author = "Hanlon, {David P.} and Watt, {David S.} and Westhead, {Edward W.}",

note = "Funding Information: 1 This investigation was supported in part, by a Public Health service (1-F2-GM-19,227-01Al) to D.P.H. from the National Institute of Arthritis bolic Diseases, and in part by grant, GM OS474 to E.W.W. 2 Imdergraduate Research Assistant, Kational lnstit,utes 225",

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doi = "10.1016/0003-2697(66)90150-3",

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AU - Hanlon, David P.

AU - Watt, David S.

AU - Westhead, Edward W.

N1 - Funding Information: 1 This investigation was supported in part, by a Public Health service (1-F2-GM-19,227-01Al) to D.P.H. from the National Institute of Arthritis bolic Diseases, and in part by grant, GM OS474 to E.W.W. 2 Imdergraduate Research Assistant, Kational lnstit,utes 225

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N2 - The interaction of 2-amino-2-(hydroxymethyl)-1,3-propanediol (tris) with eight divalent metal ions has been investigated under conditions commonly employed in biochemical studies. Formation constants have been calculated, where possible from pH titration data. Hg++ and Cu++ interact strongly with tris, and Co++, Zn++, Cd++ much less so. The interaction of tris with Mg++ and Mn++ is too weak to be detected under the conditions used. Above pH 7.5 further reaction of the metal complexes with added hydroxyl ion complicates the calculation of formation constants for higher complexes of divalent metal ions and Tris.

AB - The interaction of 2-amino-2-(hydroxymethyl)-1,3-propanediol (tris) with eight divalent metal ions has been investigated under conditions commonly employed in biochemical studies. Formation constants have been calculated, where possible from pH titration data. Hg++ and Cu++ interact strongly with tris, and Co++, Zn++, Cd++ much less so. The interaction of tris with Mg++ and Mn++ is too weak to be detected under the conditions used. Above pH 7.5 further reaction of the metal complexes with added hydroxyl ion complicates the calculation of formation constants for higher complexes of divalent metal ions and Tris.

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The interaction of divalent metal ions with tris buffer in dilute solution

Abstract

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