The interaction of divalent metal ions with tris buffer in dilute solution

David P. Hanlon, David S. Watt, Edward W. Westhead

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78 Scopus citations


The interaction of 2-amino-2-(hydroxymethyl)-1,3-propanediol (tris) with eight divalent metal ions has been investigated under conditions commonly employed in biochemical studies. Formation constants have been calculated, where possible from pH titration data. Hg++ and Cu++ interact strongly with tris, and Co++, Zn++, Cd++ much less so. The interaction of tris with Mg++ and Mn++ is too weak to be detected under the conditions used. Above pH 7.5 further reaction of the metal complexes with added hydroxyl ion complicates the calculation of formation constants for higher complexes of divalent metal ions and Tris.

Original languageEnglish
Pages (from-to)225-233
Number of pages9
JournalAnalytical Biochemistry
Issue number2
StatePublished - Aug 1966

Bibliographical note

Funding Information:
1 This investigation was supported in part, by a Public Health service (1-F2-GM-19,227-01Al) to D.P.H. from the National Institute of Arthritis bolic Diseases, and in part by grant, GM OS474 to E.W.W. 2 Imdergraduate Research Assistant, Kational lnstit,utes 225

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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