TY - JOUR
T1 - The Ketogenic Diet Increases Mitochondrial Uncoupling Protein Levels and Activity
AU - Sullivan, Patrick G.
AU - Rippy, Nancy A.
AU - Dorenbos, Kristina
AU - Concepcion, Rachele C.
AU - Agarwal, Aakash K.
AU - Rho, Jong M.
PY - 2004/4
Y1 - 2004/4
N2 - Fatty acids are known to enhance mitochondrial uncoupling protein (UCP) activity. We asked whether a highfat ketogenic diet (KD) increases UCP levels and activity in hippocampi of juvenile mice. Maximum mitochondrial respiration rates were significantly (p < 0.001) higher in KD- versus standard diet (SD)-treated animals, indicating increased UCP-mediated proton conductance that can reduce reactive oxygen species (ROS) production. Western blots showed significant (p < 0.05) or borderline significant increases in UCP2, UCP4, and UCP5 protein levels, and increased immunoreactivity to these three UCP isoforms was most prominently seen in the dentate gyrus of KD-fed mice. Finally, we found that oligomycin-induced ROS production was significantly (p < 0.05) lower in KD-fed mice than in SD controls. Collectively, our data suggest that a KD may exert neuroprotective effects by diminishing ROS production through activation of mitochondrial UCPs.
AB - Fatty acids are known to enhance mitochondrial uncoupling protein (UCP) activity. We asked whether a highfat ketogenic diet (KD) increases UCP levels and activity in hippocampi of juvenile mice. Maximum mitochondrial respiration rates were significantly (p < 0.001) higher in KD- versus standard diet (SD)-treated animals, indicating increased UCP-mediated proton conductance that can reduce reactive oxygen species (ROS) production. Western blots showed significant (p < 0.05) or borderline significant increases in UCP2, UCP4, and UCP5 protein levels, and increased immunoreactivity to these three UCP isoforms was most prominently seen in the dentate gyrus of KD-fed mice. Finally, we found that oligomycin-induced ROS production was significantly (p < 0.05) lower in KD-fed mice than in SD controls. Collectively, our data suggest that a KD may exert neuroprotective effects by diminishing ROS production through activation of mitochondrial UCPs.
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U2 - 10.1002/ana.20062
DO - 10.1002/ana.20062
M3 - Article
C2 - 15048898
AN - SCOPUS:1642289587
SN - 0364-5134
VL - 55
SP - 576
EP - 580
JO - Annals of Neurology
JF - Annals of Neurology
IS - 4
ER -