The late stage of COPI vesicle fission requires shorter forms of phosphatidic acid and diacylglycerol

Seung Yeol Park; Jia Shu Yang; Zhen Li; Pan Deng; Xiaohong Zhu; David Young; Maria Ericsson; Ruben L.H. Andringa; Adriaan J. Minnaard; Chunmei Zhu; Fei Sun; D. Branch Moody; Andrew J. Morris; Jun Fan; Victor W. Hsu

doi:10.1038/s41467-019-11324-4

The late stage of COPI vesicle fission requires shorter forms of phosphatidic acid and diacylglycerol

Seung Yeol Park, Jia Shu Yang, Zhen Li, Pan Deng, Xiaohong Zhu, David Young, Maria Ericsson, Ruben L.H. Andringa, Adriaan J. Minnaard, Chunmei Zhu, Fei Sun, D. Branch Moody, Andrew J. Morris, Jun Fan, Victor W. Hsu

Internal Medicine

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Studies on vesicle formation by the Coat Protein I (COPI) complex have contributed to a basic understanding of how vesicular transport is initiated. Phosphatidic acid (PA) and diacylglycerol (DAG) have been found previously to be required for the fission stage of COPI vesicle formation. Here, we find that PA with varying lipid geometry can all promote early fission, but only PA with shortened acyl chains promotes late fission. Moreover, diacylglycerol (DAG) acts after PA in late fission, with this role of DAG also requiring shorter acyl chains. Further highlighting the importance of the short-chain lipid geometry for late fission, we find that shorter forms of PA and DAG promote the vesiculation ability of COPI fission factors. These findings advance a general understanding of how lipid geometry contributes to membrane deformation for vesicle fission, and also how proteins and lipids coordinate their actions in driving this process.

Original language	English
Article number	3409
Journal	Nature Communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-11324-4
State	Published - Dec 1 2019

Bibliographical note

Publisher Copyright:
© 2019, The Author(s).

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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Park, S. Y., Yang, J. S., Li, Z., Deng, P., Zhu, X., Young, D., Ericsson, M., Andringa, R. L. H., Minnaard, A. J., Zhu, C., Sun, F., Moody, D. B., Morris, A. J., Fan, J., & Hsu, V. W. (2019). The late stage of COPI vesicle fission requires shorter forms of phosphatidic acid and diacylglycerol. Nature Communications, 10(1), Article 3409. https://doi.org/10.1038/s41467-019-11324-4

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abstract = "Studies on vesicle formation by the Coat Protein I (COPI) complex have contributed to a basic understanding of how vesicular transport is initiated. Phosphatidic acid (PA) and diacylglycerol (DAG) have been found previously to be required for the fission stage of COPI vesicle formation. Here, we find that PA with varying lipid geometry can all promote early fission, but only PA with shortened acyl chains promotes late fission. Moreover, diacylglycerol (DAG) acts after PA in late fission, with this role of DAG also requiring shorter acyl chains. Further highlighting the importance of the short-chain lipid geometry for late fission, we find that shorter forms of PA and DAG promote the vesiculation ability of COPI fission factors. These findings advance a general understanding of how lipid geometry contributes to membrane deformation for vesicle fission, and also how proteins and lipids coordinate their actions in driving this process.",

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AU - Park, Seung Yeol

AU - Yang, Jia Shu

AU - Li, Zhen

AU - Deng, Pan

AU - Zhu, Xiaohong

AU - Young, David

AU - Ericsson, Maria

AU - Andringa, Ruben L.H.

AU - Minnaard, Adriaan J.

AU - Zhu, Chunmei

AU - Sun, Fei

AU - Moody, D. Branch

AU - Morris, Andrew J.

AU - Fan, Jun

AU - Hsu, Victor W.

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AB - Studies on vesicle formation by the Coat Protein I (COPI) complex have contributed to a basic understanding of how vesicular transport is initiated. Phosphatidic acid (PA) and diacylglycerol (DAG) have been found previously to be required for the fission stage of COPI vesicle formation. Here, we find that PA with varying lipid geometry can all promote early fission, but only PA with shortened acyl chains promotes late fission. Moreover, diacylglycerol (DAG) acts after PA in late fission, with this role of DAG also requiring shorter acyl chains. Further highlighting the importance of the short-chain lipid geometry for late fission, we find that shorter forms of PA and DAG promote the vesiculation ability of COPI fission factors. These findings advance a general understanding of how lipid geometry contributes to membrane deformation for vesicle fission, and also how proteins and lipids coordinate their actions in driving this process.

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The late stage of COPI vesicle fission requires shorter forms of phosphatidic acid and diacylglycerol

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