The late stage of COPI vesicle fission requires shorter forms of phosphatidic acid and diacylglycerol

Seung Yeol Park; Jia Shu Yang; Zhen Li; Pan Deng; Xiaohong Zhu; David Young; Maria Ericsson; Ruben L.H. Andringa; Adriaan J. Minnaard; Chunmei Zhu; Fei Sun; D. Branch Moody; Andrew J. Morris; Jun Fan; Victor W. Hsu

doi:10.1038/s41467-019-11324-4

The late stage of COPI vesicle fission requires shorter forms of phosphatidic acid and diacylglycerol

Seung Yeol Park, Jia Shu Yang, Zhen Li, Pan Deng, Xiaohong Zhu, David Young, Maria Ericsson, Ruben L.H. Andringa, Adriaan J. Minnaard, Chunmei Zhu, Fei Sun, D. Branch Moody, Andrew J. Morris, Jun Fan, Victor W. Hsu

Internal Medicine

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Studies on vesicle formation by the Coat Protein I (COPI) complex have contributed to a basic understanding of how vesicular transport is initiated. Phosphatidic acid (PA) and diacylglycerol (DAG) have been found previously to be required for the fission stage of COPI vesicle formation. Here, we find that PA with varying lipid geometry can all promote early fission, but only PA with shortened acyl chains promotes late fission. Moreover, diacylglycerol (DAG) acts after PA in late fission, with this role of DAG also requiring shorter acyl chains. Further highlighting the importance of the short-chain lipid geometry for late fission, we find that shorter forms of PA and DAG promote the vesiculation ability of COPI fission factors. These findings advance a general understanding of how lipid geometry contributes to membrane deformation for vesicle fission, and also how proteins and lipids coordinate their actions in driving this process.

Original language	English
Article number	3409
Journal	Nature Communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-11324-4
State	Published - Dec 1 2019

Bibliographical note

Publisher Copyright:
© 2019, The Author(s).

Funding

We thank Jian Li and Jia-Wei Hsu for advice and discussions. This work was funded by grants from the U.S. National Institutes of Health to V.W.H. (GM058615), D.B.M. (AR048632, AI116604), and A.J. Morris (HL120507, P30GM127211). A.J. Morris is also funded by the U.S. Veterans Administration (I01CX001550). J.F. is funded by the Research Grants Council of Hong Kong (CityU 21300014 and CityU 11306517) and Special Program for Applied Research on Super Computation of the NSFC-Guangdong Joint Fund (the second phase) under Grant No. U1501501. A.J. Minnaard is funded by the Dutch NWO (Building Blocks of Life, 737.016.006). F.S. is funded by the Ministry of Science and Technology of China (2017YFA0504700).

Funders	Funder number
A.J. Morris	P30GM127211
Ministry of Science and Technology, China
Hong Kong Research Grants Council
Special Program for Applied Research on Super Computation of the NSFC-Guangdong Joint Fund
U.S. National Institutes of Health	GM058615
U.S. Veterans Administration
National Institutes of Health (NIH)	I01CX001550, HL120507, R37GM058615, AR048632, GM127211
National Institute of Allergy and Infectious Diseases	R01AI116604
City University of Hong Kong	21300014, 11306517
Ministry of Science and Technology of the People's Republic of China	2017YFA0504700
Nederlandse Organisatie voor Wetenschappelijk Onderzoek	737.016.006

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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Park, S. Y., Yang, J. S., Li, Z., Deng, P., Zhu, X., Young, D., Ericsson, M., Andringa, R. L. H., Minnaard, A. J., Zhu, C., Sun, F., Moody, D. B., Morris, A. J., Fan, J., & Hsu, V. W. (2019). The late stage of COPI vesicle fission requires shorter forms of phosphatidic acid and diacylglycerol. Nature Communications, 10(1), Article 3409. https://doi.org/10.1038/s41467-019-11324-4

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AU - Andringa, Ruben L.H.

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The late stage of COPI vesicle fission requires shorter forms of phosphatidic acid and diacylglycerol

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

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