The localization of centromere protein A is conserved among tissues

Eleonora Cappelletti; Francesca M. Piras; Lorenzo Sola; Marco Santagostino; Jessica L. Petersen; Rebecca R. Bellone; Carrie J. Finno; Sichong Peng; Ted S. Kalbfleisch; Ernest Bailey; Solomon G. Nergadze; Elena Giulotto

doi:10.1038/s42003-023-05335-7

The localization of centromere protein A is conserved among tissues

Eleonora Cappelletti, Francesca M. Piras, Lorenzo Sola, Marco Santagostino, Jessica L. Petersen, Rebecca R. Bellone, Carrie J. Finno, Sichong Peng, Ted S. Kalbfleisch, Ernest Bailey, Solomon G. Nergadze, Elena Giulotto

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Centromeres are epigenetically specified by the histone H3 variant CENP-A. Although mammalian centromeres are typically associated with satellite DNA, we previously demonstrated that the centromere of horse chromosome 11 (ECA11) is completely devoid of satellite DNA. We also showed that the localization of its CENP-A binding domain is not fixed but slides within an about 500 kb region in different individuals, giving rise to positional alleles. These epialleles are inherited as Mendelian traits but their position can move in one generation. It is still unknown whether centromere sliding occurs during meiosis or during development. Here, we first improve the sequence of the ECA11 centromeric region in the EquCab3.0 assembly. Then, to test whether centromere sliding may occur during development, we map the CENP-A binding domains of ECA11 using ChIP-seq in five tissues of different embryonic origin from the four horses of the equine FAANG (Functional Annotation of ANimal Genomes) consortium. Our results demonstrate that the centromere is localized in the same region in all tissues, suggesting that the position of the centromeric domain is maintained during development.

Original language	English
Article number	963
Journal	Communications Biology
Volume	6
Issue number	1
DOIs	https://doi.org/10.1038/s42003-023-05335-7
State	Published - Dec 2023

Bibliographical note

Publisher Copyright:
© 2023, Springer Nature Limited.

Funding

We would like to thank Donald Miller (Cornell University, USA) for providing us with the fibroblast cell line from Twilight and Francesco Lescai (University of Pavia) for helpful suggestions during the revision of the manuscript. This research was funded by Animal Breeding and Functional Annotation of Genomes (A1201) Grant 2019-67015-29340/Project Accession 1018854 from the USDA National Institute of Food and Agriculture and by the Italian Ministry of Education, University and Research (MIUR) (Dipartimenti di Eccellenza Program (2018–2022)—Department of Biology and Biotechnology “L. Spallanzani,” University of Pavia). The Galaxy server that was used for some calculations is in part funded by Collaborative Research Centre 992 Medical Epigenetics (DFG grant SFB 992/1 2012) and German Federal Ministry of Education and Research (BMBF grants 031 A538A/A538C RBC, 031L0101B/031L0101C de.NBI-epi, 031L0106 de.STAIR (de.NBI)).

Funders	Funder number
Collaborative Research Centre 992 Medical Epigenetics
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative
Deutsche Forschungsgemeinschaft	SFB 992/1 2012
Deutsche Forschungsgemeinschaft
Bundesministerium für Bildung und Forschung	031 A538A/A538C RBC, 031L0106 de.STAIR
Bundesministerium für Bildung und Forschung
Ministero dell’Istruzione, dell’Università e della Ricerca
Università degli Studi di Pavia

ASJC Scopus subject areas

Medicine (miscellaneous)
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences

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title = "The localization of centromere protein A is conserved among tissues",

abstract = "Centromeres are epigenetically specified by the histone H3 variant CENP-A. Although mammalian centromeres are typically associated with satellite DNA, we previously demonstrated that the centromere of horse chromosome 11 (ECA11) is completely devoid of satellite DNA. We also showed that the localization of its CENP-A binding domain is not fixed but slides within an about 500 kb region in different individuals, giving rise to positional alleles. These epialleles are inherited as Mendelian traits but their position can move in one generation. It is still unknown whether centromere sliding occurs during meiosis or during development. Here, we first improve the sequence of the ECA11 centromeric region in the EquCab3.0 assembly. Then, to test whether centromere sliding may occur during development, we map the CENP-A binding domains of ECA11 using ChIP-seq in five tissues of different embryonic origin from the four horses of the equine FAANG (Functional Annotation of ANimal Genomes) consortium. Our results demonstrate that the centromere is localized in the same region in all tissues, suggesting that the position of the centromeric domain is maintained during development.",

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AU - Bellone, Rebecca R.

AU - Finno, Carrie J.

AU - Peng, Sichong

AU - Kalbfleisch, Ted S.

AU - Bailey, Ernest

AU - Nergadze, Solomon G.

AU - Giulotto, Elena

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The localization of centromere protein A is conserved among tissues

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

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