The mouse digit tip: From wound healing to regeneration

Jennifer Simkin, Manjong Han, Ling Yu, Mingquan Yan, Ken Muneoka

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

44 Scopus citations

Abstract

A challenge to the study of regeneration is determining at what point the processes of wound healing and regeneration diverge. The mouse displays level-specific regeneration responses. An amputation through the distal third of the terminal phalanx will prompt a regeneration response and result in a new digit tip that mimics the morphology of the lost digit tip. Conversely, an amputation through the distal third of the intermediate phalanx initiates a wound healing and scarring response. The mouse, therefore, provides a model for studying the transition between wound healing and regeneration in the same animal. This chapter details the methods used in the study of mammalian digit regeneration, including a method to introduce exogenous protein into the mouse digit amputation model via microcarrier beads and methods for analysis of bone regeneration.

Original languageEnglish
Title of host publicationWound Regeneration and Repair
Subtitle of host publicationMethods and Protocols
Pages419-435
Number of pages17
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1037
ISSN (Print)1064-3745

Keywords

  • Amputation model
  • Digit regeneration
  • Scarring response

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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