The yeast Kluyveromvces lactis grows on galactose by inducing the Leloir pathway enzymes-kinase, epimerase, and transferase. To investigate the molecular mechanism for reaulating expression of this metabolic pathway we isolated GAL1 GAL7, GAL10, which code for kinase, transferase, and epimerase, respectively and characterized their size, organization, and transcriptional regulation. Our results indicate that induction of the Leloir pathway in K. lactis occurs at the level of transcription and that the organization and regulation of the GAL gene cluster in K. lactis is closely related to the homologous gene cluster in Saccharomyces cerevisiae. Likewise, the Upstream Activator Sequences that regulate induction of the GAL aenes are similar in base sequence, number and relative location in the two yeasts.
|Number of pages||18|
|Journal||Nucleic Acids Research|
|State||Published - Aug 25 1988|
Bibliographical noteFunding Information:
ACKNOWLEDGEMENTS This work was supported by Public Health Service qrant GM-22749 from the National Institutes of Health and bv Biomedical Support Grant No. RRO5374 from the Biomedical Research Suooort Branch, Division of Research Facilities and Resources. NIH. T.D.W. was supported in Dart by a fellowship from the Mason and Hanger Corporation. We thank Dr. Keith Jones for assistance with computer searches.
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