The organization and transcription of the galactose gene cluster of Kluyveromyces lactis

Thomas D. Webster; Robert C. Dickson

doi:10.1093/nar/16.16.8011

The organization and transcription of the galactose gene cluster of Kluyveromyces lactis

Thomas D. Webster, Robert C. Dickson

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The yeast Kluyveromvces lactis grows on galactose by inducing the Leloir pathway enzymes-kinase, epimerase, and transferase. To investigate the molecular mechanism for reaulating expression of this metabolic pathway we isolated GAL1 GAL7, GAL10, which code for kinase, transferase, and epimerase, respectively and characterized their size, organization, and transcriptional regulation. Our results indicate that induction of the Leloir pathway in K. lactis occurs at the level of transcription and that the organization and regulation of the GAL gene cluster in K. lactis is closely related to the homologous gene cluster in Saccharomyces cerevisiae. Likewise, the Upstream Activator Sequences that regulate induction of the GAL aenes are similar in base sequence, number and relative location in the two yeasts.

Original language	English
Pages (from-to)	8011-8028
Number of pages	18
Journal	Nucleic Acids Research
Volume	16
Issue number	16
DOIs	https://doi.org/10.1093/nar/16.16.8011
State	Published - Aug 25 1988

Bibliographical note

Funding Information:
ACKNOWLEDGEMENTS This work was supported by Public Health Service qrant GM-22749 from the National Institutes of Health and bv Biomedical Support Grant No. RRO5374 from the Biomedical Research Suooort Branch, Division of Research Facilities and Resources. NIH. T.D.W. was supported in Dart by a fellowship from the Mason and Hanger Corporation. We thank Dr. Keith Jones for assistance with computer searches.

Funding

ACKNOWLEDGEMENTS This work was supported by Public Health Service qrant GM-22749 from the National Institutes of Health and bv Biomedical Support Grant No. RRO5374 from the Biomedical Research Suooort Branch, Division of Research Facilities and Resources. NIH. T.D.W. was supported in Dart by a fellowship from the Mason and Hanger Corporation. We thank Dr. Keith Jones for assistance with computer searches.

Funders	Funder number
Mason and Hanger Corporation
National Institutes of Health (NIH)
National Institute of General Medical Sciences	R01GM022749
U.S. Public Health Service

ASJC Scopus subject areas

Genetics

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title = "The organization and transcription of the galactose gene cluster of Kluyveromyces lactis",

abstract = "The yeast Kluyveromvces lactis grows on galactose by inducing the Leloir pathway enzymes-kinase, epimerase, and transferase. To investigate the molecular mechanism for reaulating expression of this metabolic pathway we isolated GAL1 GAL7, GAL10, which code for kinase, transferase, and epimerase, respectively and characterized their size, organization, and transcriptional regulation. Our results indicate that induction of the Leloir pathway in K. lactis occurs at the level of transcription and that the organization and regulation of the GAL gene cluster in K. lactis is closely related to the homologous gene cluster in Saccharomyces cerevisiae. Likewise, the Upstream Activator Sequences that regulate induction of the GAL aenes are similar in base sequence, number and relative location in the two yeasts.",

author = "Webster, {Thomas D.} and Dickson, {Robert C.}",

note = "Funding Information: ACKNOWLEDGEMENTS This work was supported by Public Health Service qrant GM-22749 from the National Institutes of Health and bv Biomedical Support Grant No. RRO5374 from the Biomedical Research Suooort Branch, Division of Research Facilities and Resources. NIH. T.D.W. was supported in Dart by a fellowship from the Mason and Hanger Corporation. We thank Dr. Keith Jones for assistance with computer searches.",

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AU - Dickson, Robert C.

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N2 - The yeast Kluyveromvces lactis grows on galactose by inducing the Leloir pathway enzymes-kinase, epimerase, and transferase. To investigate the molecular mechanism for reaulating expression of this metabolic pathway we isolated GAL1 GAL7, GAL10, which code for kinase, transferase, and epimerase, respectively and characterized their size, organization, and transcriptional regulation. Our results indicate that induction of the Leloir pathway in K. lactis occurs at the level of transcription and that the organization and regulation of the GAL gene cluster in K. lactis is closely related to the homologous gene cluster in Saccharomyces cerevisiae. Likewise, the Upstream Activator Sequences that regulate induction of the GAL aenes are similar in base sequence, number and relative location in the two yeasts.

AB - The yeast Kluyveromvces lactis grows on galactose by inducing the Leloir pathway enzymes-kinase, epimerase, and transferase. To investigate the molecular mechanism for reaulating expression of this metabolic pathway we isolated GAL1 GAL7, GAL10, which code for kinase, transferase, and epimerase, respectively and characterized their size, organization, and transcriptional regulation. Our results indicate that induction of the Leloir pathway in K. lactis occurs at the level of transcription and that the organization and regulation of the GAL gene cluster in K. lactis is closely related to the homologous gene cluster in Saccharomyces cerevisiae. Likewise, the Upstream Activator Sequences that regulate induction of the GAL aenes are similar in base sequence, number and relative location in the two yeasts.

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The organization and transcription of the galactose gene cluster of Kluyveromyces lactis

Abstract

Bibliographical note

Funding

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