Abstract
Backbone-breaking oxygenase clusters. Inactivation of the oxygenase-encoding genes jadH and jadG and structure elucidation of novel accumulation products illustrate the essential interactions within the biosynthetic protein complex that govern the oxygenation cascade leading to the cleavage of the angucycline backbone during jadomycin biosynthesis by Streptomyces venezuelae. These results also allow the elucidation of the dehydration sequence (shown) that yields the aromatic ring A of the jadomycin scaffold.
Original language | English |
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Pages (from-to) | 838-845 |
Number of pages | 8 |
Journal | ChemBioChem |
Volume | 6 |
Issue number | 5 |
DOIs | |
State | Published - May 2005 |
Keywords
- Biosynthesis
- Cleavage reactions
- Dehydratases
- Oxygenases
- Polyketides
- Proteins
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Organic Chemistry