The oxidative ring cleavage in jadomycin biosynthesis: A multistep oxygenation cascade in a biosynthetic black box

Uwe Rix; Chenchen Wang; Yihua Chen; Fredilyn M. Lipata; Lily L.Remsing Rix; Lisa M. Greenwell; Leo C. Vining; Keqian Yang; Jürgen Rohr

doi:10.1002/cbic.200400395

The oxidative ring cleavage in jadomycin biosynthesis: A multistep oxygenation cascade in a biosynthetic black box

Uwe Rix, Chenchen Wang, Yihua Chen, Fredilyn M. Lipata, Lily L.Remsing Rix, Lisa M. Greenwell, Leo C. Vining, Keqian Yang, Jürgen Rohr

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

Backbone-breaking oxygenase clusters. Inactivation of the oxygenase-encoding genes jadH and jadG and structure elucidation of novel accumulation products illustrate the essential interactions within the biosynthetic protein complex that govern the oxygenation cascade leading to the cleavage of the angucycline backbone during jadomycin biosynthesis by Streptomyces venezuelae. These results also allow the elucidation of the dehydration sequence (shown) that yields the aromatic ring A of the jadomycin scaffold.

Original language	English
Pages (from-to)	838-845
Number of pages	8
Journal	ChemBioChem
Volume	6
Issue number	5
DOIs	https://doi.org/10.1002/cbic.200400395
State	Published - May 2005

Keywords

Biosynthesis
Cleavage reactions
Dehydratases
Oxygenases
Polyketides
Proteins

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

Access to Document

10.1002/cbic.200400395

Cite this

@article{a387273aaf1f42b79ec3d8ff50e2db0c,

title = "The oxidative ring cleavage in jadomycin biosynthesis: A multistep oxygenation cascade in a biosynthetic black box",

abstract = "Backbone-breaking oxygenase clusters. Inactivation of the oxygenase-encoding genes jadH and jadG and structure elucidation of novel accumulation products illustrate the essential interactions within the biosynthetic protein complex that govern the oxygenation cascade leading to the cleavage of the angucycline backbone during jadomycin biosynthesis by Streptomyces venezuelae. These results also allow the elucidation of the dehydration sequence (shown) that yields the aromatic ring A of the jadomycin scaffold.",

keywords = "Biosynthesis, Cleavage reactions, Dehydratases, Oxygenases, Polyketides, Proteins",

author = "Uwe Rix and Chenchen Wang and Yihua Chen and Lipata, {Fredilyn M.} and Rix, {Lily L.Remsing} and Greenwell, {Lisa M.} and Vining, {Leo C.} and Keqian Yang and J{\"u}rgen Rohr",

year = "2005",

month = may,

doi = "10.1002/cbic.200400395",

language = "English",

volume = "6",

pages = "838--845",

number = "5",

}

TY - JOUR

T1 - The oxidative ring cleavage in jadomycin biosynthesis

T2 - A multistep oxygenation cascade in a biosynthetic black box

AU - Rix, Uwe

AU - Wang, Chenchen

AU - Chen, Yihua

AU - Lipata, Fredilyn M.

AU - Rix, Lily L.Remsing

AU - Greenwell, Lisa M.

AU - Vining, Leo C.

AU - Yang, Keqian

AU - Rohr, Jürgen

PY - 2005/5

Y1 - 2005/5

N2 - Backbone-breaking oxygenase clusters. Inactivation of the oxygenase-encoding genes jadH and jadG and structure elucidation of novel accumulation products illustrate the essential interactions within the biosynthetic protein complex that govern the oxygenation cascade leading to the cleavage of the angucycline backbone during jadomycin biosynthesis by Streptomyces venezuelae. These results also allow the elucidation of the dehydration sequence (shown) that yields the aromatic ring A of the jadomycin scaffold.

AB - Backbone-breaking oxygenase clusters. Inactivation of the oxygenase-encoding genes jadH and jadG and structure elucidation of novel accumulation products illustrate the essential interactions within the biosynthetic protein complex that govern the oxygenation cascade leading to the cleavage of the angucycline backbone during jadomycin biosynthesis by Streptomyces venezuelae. These results also allow the elucidation of the dehydration sequence (shown) that yields the aromatic ring A of the jadomycin scaffold.

KW - Biosynthesis

KW - Cleavage reactions

KW - Dehydratases

KW - Oxygenases

KW - Polyketides

KW - Proteins

UR - http://www.scopus.com/inward/record.url?scp=20444493372&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20444493372&partnerID=8YFLogxK

U2 - 10.1002/cbic.200400395

DO - 10.1002/cbic.200400395

M3 - Article

C2 - 15776503

AN - SCOPUS:20444493372

SN - 1439-4227

VL - 6

SP - 838

EP - 845

JO - ChemBioChem

JF - ChemBioChem

IS - 5

ER -

The oxidative ring cleavage in jadomycin biosynthesis: A multistep oxygenation cascade in a biosynthetic black box

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this