Abstract
The paramyxovirus fusion (F) protein mediates membrane fusion. The biologically active F protein consists of a membrane distal subunit F2 and a membrane anchored subunit F1. A highly stable structure has been identified comprised of peptides derived from the simian virus 5 (SV5) F1 heptad repeat A, which abuts the hydrophobic fusion peptide (peptide N-1), and the SV5 F1 heptad repeat B, located 270 residues downstream and adjacent to the transmembrane domain (peptides C-1 and C-2). In isolation, peptide N-1 is 47% α-helical and peptide C-1 and C-2 are unfolded. When mixed together, peptides N1+C1 form a thermostable (T(m) > 90°C), 82% α-helical, discrete trimer of heterodimers (mass 31,300 M(r)) that is resistant to denaturation by 2% SDS at 40°C. The authors suggest that this α-helical trimeric complex represents the core most stable form of the F protein that is either fusion competent or forms after fusion has occurred. Peptide C-1 is a potent inhibitor of both the lipid mixing and aqueous content mixing fusion activity of the SV5 F protein. In contrast, peptide N-1 inhibits cytoplasmic content mixing but not lipid mixing, leading to a stable hemifusion state. Thus, these peptides define functionally different steps in the fusion process. The parallels among both the fusion processes and the protein structures of paramyxovirus F proteins, HIV gp41 and influenza virus haemagglutinin are discussed, as the analogies are indicative of a conserved paradigm for fusion promotion among fusion proteins from widely disparate viruses.
Original language | English |
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Pages (from-to) | 11-19 |
Number of pages | 9 |
Journal | Molecular Membrane Biology |
Volume | 16 |
Issue number | 1 |
DOIs | |
State | Published - 1999 |
Bibliographical note
Copyright:Copyright 2017 Elsevier B.V., All rights reserved.
Keywords
- Fusion
- Membranes
- Paramyxovirus
- Peptides
- Viruses
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology