The pleiotropic role of the 26S proteasome subunit RPN10 in Arabidopsis growth and development supports a substrate-specific function in abscisic acid signaling

Jan Smalle, Jasmina Kurepa, Peizhen Yang, Thomas J. Emborg, Elena Babiychuk, Sergei Kushnir, Richard D. Vierstra

Research output: Contribution to journalArticlepeer-review

223 Scopus citations

Abstract

The 26S proteasome is an essential protease complex responsible for removing most short-lived intracellular proteins, especially those modified with polyubiquitin chains. We show here that an Arabidopsis mutant expressing an altered RPN10 subunit exhibited a pleiotropic phenotype consistent with specific changes in 26S proteasome function. rpn10-1 plants displayed reduced seed germination, growth rate, stamen number, genetic transmission through the male gamete, and hormone-induced cell division, which can be explained partially by a constitutive downregulation of the key cell cycle gene CDKA;1. rpn10-1 also was more sensitive to abscisic acid (ABA), salt, and sucrose stress and to DNA-damaging agents and had decreased sensitivity to cytokinin and auxin. Most of the phenotypes can be explained by a hypersensitivity to ABA, which is reflected at the molecular level by the selective stabilization of the short-lived ABA-signaling protein ABI5. Collectively, these results indicate that RPN10 affects a number of regulatory processes in Arabidopsis likely by directing specific proteins to the 26S proteasome for degradation. A particularly important role may be in regulating the responses to signals promulgated by ABA.

Original languageEnglish
Pages (from-to)965-980
Number of pages16
JournalPlant Cell
Volume15
Issue number4
DOIs
StatePublished - Apr 1 2003

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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