The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses

Barbara Téllez-Robledo, Concepcion Manzano, Angela Saez, Sara Navarro-Neila, Javier Silva-Navas, Laura de Lorenzo, Mary Paz González-García, René Toribio, Arthur G. Hunt, Roberto Baigorri, Ilda Casimiro, Siobhan M. Brady, M. Mar Castellano, J. Carlos del Pozo

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include changes in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA is important for root development and responses. Mutations in FIP1, a component of polyadenylation machinery, affects plant development, cell division and elongation, and response to different abiotic stresses. Salt treatment increases the amount of poly(A) site usage within the coding region and 5′ untranslated regions (5′-UTRs), and the lack of FIP1 activity reduces the poly(A) site usage within these non-canonical sites. Gene ontology analyses of transcripts displaying APA in response to salt show an enrichment in ABA signaling, and in the response to stresses such as salt or cadmium (Cd), among others. Root growth assays show that fip1-2 is more tolerant to salt but is hypersensitive to ABA or Cd. Our data indicate that FIP1-mediated alternative polyadenylation is important for plant development and stress responses.

Original languageEnglish
Pages (from-to)1203-1219
Number of pages17
JournalPlant Journal
Volume99
Issue number6
DOIs
StatePublished - Sep 1 2019

Bibliographical note

Funding Information:
This research was supported by grants from the Spanish Government (BIO2017-82209-R and BIO2014-52091-R to J.C.P.) and by the "Severo Ochoa Program for Centres of Excellence in R&D” from the Agencia Estatal de Investigación of Spain (grant SEV-2016-0672 (2017-2021)) to the CBGP. B.T. was supported by a predoctoral fellowship (BES-2012-054056) from MINECO (Spain). C.M. was supported by a Marie Skłodowska-Curie fellowship (Root Barriers 655406) from the European Commission. S.M.B is supported by an Howard Hughes Medical Institute (HHMI) Faculty Scholar Fellowship. We thank J.L. Micol and M.R. Ponce for mapping the fip1-2 mutation, and Jose Manuel Franco and Genomic4all for help with the RNAseq and biocomputational analyses. We also thank Mark Estelle for kindly providing us with the DR5:GFP seeds.

Publisher Copyright:
© 2019 The Authors The Plant Journal © 2019 John Wiley & Sons Ltd

Keywords

  • Arabidopsis
  • FIP1
  • alternative polyadenylation
  • root development
  • stem cell maintenance
  • stress responses

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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