The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses

Barbara Téllez-Robledo; Concepcion Manzano; Angela Saez; Sara Navarro-Neila; Javier Silva-Navas; Laura de Lorenzo; Mary Paz González-García; René Toribio; Arthur G. Hunt; Roberto Baigorri; Ilda Casimiro; Siobhan M. Brady; M. Mar Castellano; J. Carlos del Pozo

doi:10.1111/tpj.14416

The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses

Barbara Téllez-Robledo, Concepcion Manzano, Angela Saez, Sara Navarro-Neila, Javier Silva-Navas, Laura de Lorenzo, Mary Paz González-García, René Toribio, Arthur G. Hunt, Roberto Baigorri, Ilda Casimiro, Siobhan M. Brady, M. Mar Castellano, J. Carlos del Pozo

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include changes in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA is important for root development and responses. Mutations in FIP1, a component of polyadenylation machinery, affects plant development, cell division and elongation, and response to different abiotic stresses. Salt treatment increases the amount of poly(A) site usage within the coding region and 5′ untranslated regions (5′-UTRs), and the lack of FIP1 activity reduces the poly(A) site usage within these non-canonical sites. Gene ontology analyses of transcripts displaying APA in response to salt show an enrichment in ABA signaling, and in the response to stresses such as salt or cadmium (Cd), among others. Root growth assays show that fip1-2 is more tolerant to salt but is hypersensitive to ABA or Cd. Our data indicate that FIP1-mediated alternative polyadenylation is important for plant development and stress responses.

Original language	English
Pages (from-to)	1203-1219
Number of pages	17
Journal	Plant Journal
Volume	99
Issue number	6
DOIs	https://doi.org/10.1111/tpj.14416
State	Published - Sep 1 2019

Bibliographical note

Publisher Copyright:
© 2019 The Authors The Plant Journal © 2019 John Wiley & Sons Ltd

Funding

This research was supported by grants from the Spanish Government (BIO2017-82209-R and BIO2014-52091-R to J.C.P.) and by the "Severo Ochoa Program for Centres of Excellence in R&D” from the Agencia Estatal de Investigación of Spain (grant SEV-2016-0672 (2017-2021)) to the CBGP. B.T. was supported by a predoctoral fellowship (BES-2012-054056) from MINECO (Spain). C.M. was supported by a Marie Skłodowska-Curie fellowship (Root Barriers 655406) from the European Commission. S.M.B is supported by an Howard Hughes Medical Institute (HHMI) Faculty Scholar Fellowship. We thank J.L. Micol and M.R. Ponce for mapping the fip1-2 mutation, and Jose Manuel Franco and Genomic4all for help with the RNAseq and biocomputational analyses. We also thank Mark Estelle for kindly providing us with the DR5:GFP seeds.

Funders	Funder number
Marie Skłodowska-Curie fellowship	655406
Spanish government	BIO2014-52091-R, BIO2017-82209-R
Howard Hughes Medical Institute
European Commission
Ministerio de Economía y Competitividad
Agencia Estatal de Investigación	BES-2012-054056, SEV-2016-0672

Keywords

Arabidopsis
FIP1
alternative polyadenylation
root development
stem cell maintenance
stress responses

ASJC Scopus subject areas

Genetics
Plant Science
Cell Biology

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10.1111/tpj.14416

Cite this

Téllez-Robledo, B., Manzano, C., Saez, A., Navarro-Neila, S., Silva-Navas, J., de Lorenzo, L., González-García, M. P., Toribio, R., Hunt, A. G., Baigorri, R., Casimiro, I., Brady, S. M., Castellano, M. M., & del Pozo, J. C. (2019). The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses. Plant Journal, 99(6), 1203-1219. https://doi.org/10.1111/tpj.14416

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abstract = "Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include changes in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA is important for root development and responses. Mutations in FIP1, a component of polyadenylation machinery, affects plant development, cell division and elongation, and response to different abiotic stresses. Salt treatment increases the amount of poly(A) site usage within the coding region and 5′ untranslated regions (5′-UTRs), and the lack of FIP1 activity reduces the poly(A) site usage within these non-canonical sites. Gene ontology analyses of transcripts displaying APA in response to salt show an enrichment in ABA signaling, and in the response to stresses such as salt or cadmium (Cd), among others. Root growth assays show that fip1-2 is more tolerant to salt but is hypersensitive to ABA or Cd. Our data indicate that FIP1-mediated alternative polyadenylation is important for plant development and stress responses.",

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Téllez-Robledo, B, Manzano, C, Saez, A, Navarro-Neila, S, Silva-Navas, J, de Lorenzo, L, González-García, MP, Toribio, R, Hunt, AG, Baigorri, R, Casimiro, I, Brady, SM, Castellano, MM & del Pozo, JC 2019, 'The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses', Plant Journal, vol. 99, no. 6, pp. 1203-1219. https://doi.org/10.1111/tpj.14416

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AU - Navarro-Neila, Sara

AU - Silva-Navas, Javier

AU - de Lorenzo, Laura

AU - González-García, Mary Paz

AU - Toribio, René

AU - Hunt, Arthur G.

AU - Baigorri, Roberto

AU - Casimiro, Ilda

AU - Brady, Siobhan M.

AU - Castellano, M. Mar

AU - del Pozo, J. Carlos

PY - 2019/9/1

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AB - Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include changes in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA is important for root development and responses. Mutations in FIP1, a component of polyadenylation machinery, affects plant development, cell division and elongation, and response to different abiotic stresses. Salt treatment increases the amount of poly(A) site usage within the coding region and 5′ untranslated regions (5′-UTRs), and the lack of FIP1 activity reduces the poly(A) site usage within these non-canonical sites. Gene ontology analyses of transcripts displaying APA in response to salt show an enrichment in ABA signaling, and in the response to stresses such as salt or cadmium (Cd), among others. Root growth assays show that fip1-2 is more tolerant to salt but is hypersensitive to ABA or Cd. Our data indicate that FIP1-mediated alternative polyadenylation is important for plant development and stress responses.

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The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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