The proliferation of human tumor cell lines in the presence of different agars, agaroses, and methyl cellulose

Edward J. Pavlik; Daniel E. Kenady; John R. van Nagell; Kathryn Keaton; Elvis S. Donaldson; Michael B. Hanson; Robert C. Flanigan

doi:10.1007/BF02619602

The proliferation of human tumor cell lines in the presence of different agars, agaroses, and methyl cellulose

Edward J. Pavlik, Daniel E. Kenady, John R. van Nagell, Kathryn Keaton, Elvis S. Donaldson, Michael B. Hanson, Robert C. Flanigan

Obstetrics and Gynecology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Human tumor cell lines, derived from cancers of the colon, ovary, and cervix, were grown in liquid tissue culture media and media made semisolid with agar (Bacto & deoxycholate lactose agar), agarose [LE, ME, Sea Plaque and Sea Prep (15/45)], and methyl cellulose. The effects of each agent on overall cell proliferation and rate of overall cell proliferation were examined. The agents, used to make media semisolid, were observed to inhibit or, in some cases, enhance cell growth in a fashion that was characteristic of individual cell lines. These phenomena may be of consequence to the optimization of nutrient media for primary tumor cell preparations.

Original language	English
Pages (from-to)	538-550
Number of pages	13
Journal	In Vitro
Volume	19
Issue number	7
DOIs	https://doi.org/10.1007/BF02619602
State	Published - Jul 1983

Keywords

agaroses
agars
methyl cellulose
proliferation
tumor cell lines

ASJC Scopus subject areas

Developmental Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF02619602

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title = "The proliferation of human tumor cell lines in the presence of different agars, agaroses, and methyl cellulose",

abstract = "Human tumor cell lines, derived from cancers of the colon, ovary, and cervix, were grown in liquid tissue culture media and media made semisolid with agar (Bacto & deoxycholate lactose agar), agarose [LE, ME, Sea Plaque and Sea Prep (15/45)], and methyl cellulose. The effects of each agent on overall cell proliferation and rate of overall cell proliferation were examined. The agents, used to make media semisolid, were observed to inhibit or, in some cases, enhance cell growth in a fashion that was characteristic of individual cell lines. These phenomena may be of consequence to the optimization of nutrient media for primary tumor cell preparations.",

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T1 - The proliferation of human tumor cell lines in the presence of different agars, agaroses, and methyl cellulose

AU - Pavlik, Edward J.

AU - Kenady, Daniel E.

AU - van Nagell, John R.

AU - Keaton, Kathryn

AU - Donaldson, Elvis S.

AU - Hanson, Michael B.

AU - Flanigan, Robert C.

PY - 1983/7

Y1 - 1983/7

N2 - Human tumor cell lines, derived from cancers of the colon, ovary, and cervix, were grown in liquid tissue culture media and media made semisolid with agar (Bacto & deoxycholate lactose agar), agarose [LE, ME, Sea Plaque and Sea Prep (15/45)], and methyl cellulose. The effects of each agent on overall cell proliferation and rate of overall cell proliferation were examined. The agents, used to make media semisolid, were observed to inhibit or, in some cases, enhance cell growth in a fashion that was characteristic of individual cell lines. These phenomena may be of consequence to the optimization of nutrient media for primary tumor cell preparations.

AB - Human tumor cell lines, derived from cancers of the colon, ovary, and cervix, were grown in liquid tissue culture media and media made semisolid with agar (Bacto & deoxycholate lactose agar), agarose [LE, ME, Sea Plaque and Sea Prep (15/45)], and methyl cellulose. The effects of each agent on overall cell proliferation and rate of overall cell proliferation were examined. The agents, used to make media semisolid, were observed to inhibit or, in some cases, enhance cell growth in a fashion that was characteristic of individual cell lines. These phenomena may be of consequence to the optimization of nutrient media for primary tumor cell preparations.

KW - agaroses

KW - agars

KW - methyl cellulose

KW - proliferation

KW - tumor cell lines

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The proliferation of human tumor cell lines in the presence of different agars, agaroses, and methyl cellulose

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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