TY - JOUR
T1 - The rise and rise of nicotiana benthamiana
T2 - A plant for all reasons
AU - Bally, Julia
AU - Jung, Hyungtaek
AU - Mortimer, Cara
AU - Naim, Fatima
AU - Philips, Joshua G.
AU - Hellens, Roger
AU - Bombarely, Aureliano
AU - Goodin, Michael M.
AU - Waterhouse, Peter M.
N1 - Publisher Copyright:
Copyright © 2018 by Annual Reviews. All rights reserved.
PY - 2018/8/25
Y1 - 2018/8/25
N2 - A decade ago, the value of Nicotiana benthamiana as a tool for plant molecular biologists was beginning to be appreciated. Scientists were using it to study plant-microbe and protein-protein interactions, and it was the species of choice with which to activate plasmid-encoded viruses, screen for gene functions with virus-induced gene silencing (VIGS), and transiently express genes by leaf agroinfiltration. However, little information about the species' origin, diversity, genetics, and genomics was available, and biologists were asking the question of whether N. benthamiana is a second fiddle or virtuoso. In this review, we look at the increased knowledge about the species and its applications over the past decade. Although N. benthamiana may still be the sidekick to Arabidopsis, it shines ever more brightly with realized and yet-to-be-exploited potential.
AB - A decade ago, the value of Nicotiana benthamiana as a tool for plant molecular biologists was beginning to be appreciated. Scientists were using it to study plant-microbe and protein-protein interactions, and it was the species of choice with which to activate plasmid-encoded viruses, screen for gene functions with virus-induced gene silencing (VIGS), and transiently express genes by leaf agroinfiltration. However, little information about the species' origin, diversity, genetics, and genomics was available, and biologists were asking the question of whether N. benthamiana is a second fiddle or virtuoso. In this review, we look at the increased knowledge about the species and its applications over the past decade. Although N. benthamiana may still be the sidekick to Arabidopsis, it shines ever more brightly with realized and yet-to-be-exploited potential.
KW - Nicotiana benthamiana
KW - VIGS
KW - agroinfiltration
KW - biofactory
KW - cellular localization
KW - polyploid genome
KW - virus-induced gene silencing
UR - http://www.scopus.com/inward/record.url?scp=85053006028&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85053006028&partnerID=8YFLogxK
U2 - 10.1146/annurev-phyto-080417-050141
DO - 10.1146/annurev-phyto-080417-050141
M3 - Review article
C2 - 30149789
AN - SCOPUS:85053006028
SN - 0066-4286
VL - 56
SP - 405
EP - 426
JO - Annual Review of Phytopathology
JF - Annual Review of Phytopathology
ER -