The role for runt related transcription factor 2 (RUNX2) as a transcriptional repressor in luteinizing granulosa cells

Eun Sil Park, Jiyeon Park, Renny T. Franceschi, Misung Jo

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Transcription factors induced by the LH surge play a vital role in reprogramming the gene expression in periovulatory follicles. The present study investigated the role of RUNX2 transcription factor in regulating the expression of Runx1, Ptgs2, and Tnfaip6 using cultured granulosa cells isolated from PMSG-primed immature rats. hCG or forskolin+PMA induced the transient increase in Runx1, Ptgs2, and Tnfaip6 expression, while the expression of Runx2 continued to increase until 48h. The knockdown of the agonist-stimulated Runx2 expression increased Runx1, Ptgs2, and Tnfaip6 expression and PGE2 levels in luteinizing granulosa cells. Conversely, the over-expression of RUNX2 inhibited the expression of these genes and PGE2 levels. The mutation of RUNX binding motifs in the Runx1 promoter enhanced transcriptional activity of the Runx1 promoter. The knockdown and overexpression of Runx2 increased and decreased Runx1 promoter activity, respectively. ChIP assays revealed the binding of RUNX2 in the Runx1 and Ptgs2 promoters. Together, these novel findings provide support for the role of RUNX2 in down-regulation of Runx1, Ptgs2, and Tnfaip6 during the late ovulatory period to support proper ovulation and/or luteinization.

Original languageEnglish
Pages (from-to)165-175
Number of pages11
JournalMolecular and Cellular Endocrinology
Issue number1-2
StatePublished - Oct 15 2012

Bibliographical note

Funding Information:
This work was supported by National Institutes of Health Grants by R01HD061617 and R03HD066012 (to M.J.).


  • Granulosa cells
  • LH
  • Ptgs2
  • Runx1
  • Runx2
  • Tnfaip6

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology


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