The role of phosphatidylinositol-3 kinase in vanadate-promoted S phase entry

Zhuo Zhang, Ning Gao, Hengjun He, Chuanshu Huang, Bing Hua Jiang, Luo Jia, Xianglin Shi

Research output: Contribution to journalReview articlepeer-review

5 Scopus citations


Phosphatidylinositil-3 kinase (PI3K) is a heterodimer of catalytic and regulatory subunits. It is involved in various signaling pathways and key functions of the cells. The present study investigated the role of PI3K in vanadate-induced alteration in cell cycle regulation in C141 mouse epidermal cells. Vanadate caused a time- and dose-dependent increase in PI3K activity and phosphorylation of p70 S6 kinase (p70S6K) at Thr421/Ser424 and Thr389 sites. The phosphorylation at these sites was inhibited by PI3K inhibitor, LY294002, and p70S6K mutation. Vanadate promoted S phase entry and this promotion was inhibited by LY294002 and rapmycin, a p70S6K inhibitor. Vanadate-induced enhancement in S phase entry was also inhibited in transfection with dominant negative p70S6K mutant cells. The results obtained show that vanadate is able to increase PI3K activity through phosphorylation. PI3K activated p70S6K, which phosphated protein S6, and promoted S phase entry.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalMolecular and Cellular Biochemistry
Issue number1-2
StatePublished - Jan 2004


  • Cell cycle regulation
  • PI3-K
  • Signaling pathway
  • Vanadate
  • p70 S6 kinase

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology


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