TY - JOUR
T1 - The role of tandem acyl carrier protein domains in polyunsaturated fatty acid biosynthesis
AU - Jiang, Hui
AU - Zirkle, Ross
AU - Metz, James G.
AU - Braun, Lisa
AU - Richter, Leslie
AU - Van Lanen, Steven G.
AU - Shen, Ben
PY - 2008/5/21
Y1 - 2008/5/21
N2 - Acyl carrier protein (ACP) plays an essential role in fatty acid and polyketide biosynthesis, and most of the fatty acid synthases (FASs) and polyketide synthases (PKSs) known to date are characterized with a single ACP for each cycle of chain elongation. Polyunsaturated fatty acid (PUFA) biosynthesis is catalyzed by the PUFA synthase, and all PUFA synthases known to date contain tandem ACPs (ranging from 5 to 9). Using the Pfa PUFA synthase from Shewanella japonica as a model system, we report here that these tandem ACPs are functionally equivalent regardless of their physical location within the PUFA synthase subunit, but the total number of ACPs controls the overall PUFA titer. These findings set the stage to interrogate other domains and subunits of PUFA synthase for their roles in controlling the final PUFA products and could potentially be exploited to improve PUFA production.
AB - Acyl carrier protein (ACP) plays an essential role in fatty acid and polyketide biosynthesis, and most of the fatty acid synthases (FASs) and polyketide synthases (PKSs) known to date are characterized with a single ACP for each cycle of chain elongation. Polyunsaturated fatty acid (PUFA) biosynthesis is catalyzed by the PUFA synthase, and all PUFA synthases known to date contain tandem ACPs (ranging from 5 to 9). Using the Pfa PUFA synthase from Shewanella japonica as a model system, we report here that these tandem ACPs are functionally equivalent regardless of their physical location within the PUFA synthase subunit, but the total number of ACPs controls the overall PUFA titer. These findings set the stage to interrogate other domains and subunits of PUFA synthase for their roles in controlling the final PUFA products and could potentially be exploited to improve PUFA production.
UR - http://www.scopus.com/inward/record.url?scp=43949138588&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=43949138588&partnerID=8YFLogxK
U2 - 10.1021/ja801911t
DO - 10.1021/ja801911t
M3 - Article
C2 - 18444614
AN - SCOPUS:43949138588
SN - 0002-7863
VL - 130
SP - 6336
EP - 6337
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 20
ER -