RNA viruses are the champions of evolution due to high frequency mutations and genetic recombination occurring during virus replication. These genetic events are due to the error-prone nature of viral RNA-dependent RNA polymerases (RdRp). Recently emerging models on viral RNA recombination, however, also include key roles for host and environmental factors. Accordingly, genome-wide screens and global proteomics approaches with Tomato bushy stunt virus (TBSV) and yeast (Saccharomyces cerevisiae) as a model host have identified 38 host proteins affecting viral RNA recombination. Follow-up studies have identified key host proteins and cellular pathways involved in TBSV RNA recombination. In addition, environmental factors, such as salt stress, have been shown to affect TBSV recombination via influencing key host or viral factors involved in the recombination process. These advances will help build more accurate models on viral recombination, evolution, and adaptation.
|Title of host publication||Advances in Virus Research|
|Number of pages||22|
|State||Published - 2011|
|Name||Advances in Virus Research|
Bibliographical noteFunding Information:
We are grateful to Dr. Hannah Jaag for helpful discussions. This work was supported by NSF (MCB0078152), NIH (5R21A1072170), and by the University of Kentucky to P. D. N.
- Environmental factors
- Host factors genome-wide screens
- RNA Recombination
- Tomato bushy stunt virus
- Viral replicase complex
- Virus replication
- Yeast as a host
ASJC Scopus subject areas
- Infectious Diseases