The sea urchin stem-loop-binding protein: A maternally expressed protein that probably functions in expression of multiple classes of histone mRNA

Anthony J. Robertson; Jason T. Howard; Zbigniew Dominski; Bradley J. Schnackenberg; Jan L. Sumerel; John J. McCarthy; James A. Coffman; William F. Marzluff

doi:10.1093/nar/gkh193

The sea urchin stem-loop-binding protein: A maternally expressed protein that probably functions in expression of multiple classes of histone mRNA

Anthony J. Robertson, Jason T. Howard, Zbigniew Dominski, Bradley J. Schnackenberg, Jan L. Sumerel, John J. McCarthy, James A. Coffman, William F. Marzluff

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Following the completion of oogenesis and oocyte maturation, histone mRNAs are synthesized and stored in the sea urchin egg pronucleus. Histone mRNAs are the only mRNAs that are not polyadenylated but instead end in a stem-loop which has been conserved in evolution. The 3′ end binds the stem-loop-binding protein (SLBP), and SLBP is required for histone pre-mRNA processing as well as translation of the histone mRNAs. A cDNA encoding a 59 kDa sea urchin SLBP (suSLBP) has been cloned from an oocyte cDNA library. The suSLBP contains an RNA-binding domain that is similar to the RNA-binding domain found in SLBPs from other species, although there is no similarity between the rest of the suSLBP and other SLBPs. The suSLBP is present at constant levels in eggs and for the first 12 h of development. The levels of suSLBP then decline and remain at a low level for the rest of embryogenesis. The suSLBP is concentrated in the egg pronucleus and is released from the nucleus only when cells enter the first mitosis. SuSLBP expressed by in vitro translation does not bind the stem-loop RNA, suggesting that suSLBP is modified to activate RNA binding in sea urchin embryos.

Original language	English
Pages (from-to)	811-818
Number of pages	8
Journal	Nucleic Acids Research
Volume	32
Issue number	2
DOIs	https://doi.org/10.1093/nar/gkh193
State	Published - 2004

Bibliographical note

Funding Information:
We thank Dr Hemant Kelkar of the UNC Bioinformatics Center for assistance in the sea urchin genome analysis. This work was supported by NIH grants GM58961 to W.F.M. and F32GM20151 to B.J.S., and by the Stowers Foundation for Medical Research (J.A.C.).

Funding

We thank Dr Hemant Kelkar of the UNC Bioinformatics Center for assistance in the sea urchin genome analysis. This work was supported by NIH grants GM58961 to W.F.M. and F32GM20151 to B.J.S., and by the Stowers Foundation for Medical Research (J.A.C.).

Funders	Funder number
National Institutes of Health (NIH)	GM58961
National Institute of General Medical Sciences	F32GM020151
Stowers Institute for Medical Research

ASJC Scopus subject areas

Genetics

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title = "The sea urchin stem-loop-binding protein: A maternally expressed protein that probably functions in expression of multiple classes of histone mRNA",

abstract = "Following the completion of oogenesis and oocyte maturation, histone mRNAs are synthesized and stored in the sea urchin egg pronucleus. Histone mRNAs are the only mRNAs that are not polyadenylated but instead end in a stem-loop which has been conserved in evolution. The 3′ end binds the stem-loop-binding protein (SLBP), and SLBP is required for histone pre-mRNA processing as well as translation of the histone mRNAs. A cDNA encoding a 59 kDa sea urchin SLBP (suSLBP) has been cloned from an oocyte cDNA library. The suSLBP contains an RNA-binding domain that is similar to the RNA-binding domain found in SLBPs from other species, although there is no similarity between the rest of the suSLBP and other SLBPs. The suSLBP is present at constant levels in eggs and for the first 12 h of development. The levels of suSLBP then decline and remain at a low level for the rest of embryogenesis. The suSLBP is concentrated in the egg pronucleus and is released from the nucleus only when cells enter the first mitosis. SuSLBP expressed by in vitro translation does not bind the stem-loop RNA, suggesting that suSLBP is modified to activate RNA binding in sea urchin embryos.",

author = "Robertson, {Anthony J.} and Howard, {Jason T.} and Zbigniew Dominski and Schnackenberg, {Bradley J.} and Sumerel, {Jan L.} and McCarthy, {John J.} and Coffman, {James A.} and Marzluff, {William F.}",

note = "Funding Information: We thank Dr Hemant Kelkar of the UNC Bioinformatics Center for assistance in the sea urchin genome analysis. This work was supported by NIH grants GM58961 to W.F.M. and F32GM20151 to B.J.S., and by the Stowers Foundation for Medical Research (J.A.C.).",

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AU - Schnackenberg, Bradley J.

AU - Sumerel, Jan L.

AU - McCarthy, John J.

AU - Coffman, James A.

AU - Marzluff, William F.

N1 - Funding Information: We thank Dr Hemant Kelkar of the UNC Bioinformatics Center for assistance in the sea urchin genome analysis. This work was supported by NIH grants GM58961 to W.F.M. and F32GM20151 to B.J.S., and by the Stowers Foundation for Medical Research (J.A.C.).

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The sea urchin stem-loop-binding protein: A maternally expressed protein that probably functions in expression of multiple classes of histone mRNA

Abstract

Bibliographical note

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