The selective pathway and a high-density lipoprotein receptor (SR-BI) in ovarian granulosa cells of the mouse

Eve Reaven, Ya Lua, Ann Nomoto, Ryan Temel, David L. Williams, Deneys R. Van Der Westhuyzen, Salman Azhar

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

We recently reported that rat luteinized ovary tissue and primary cultures of rat ovarian granulosa cells reveal a remarkably tight functional correlation between expressed selective uptake of lipoprotein cholesteryl esters and the expression of an HDL receptor protein, scavenger receptor, class B, type I (SR-BI). In the current study, we examine these same processes in C57 mouse granulosa cells and report a different correlation. Unlike the rat cells, non-hormone stimulated mouse granulosa cells are able to effectively carry out their selective pathway functions and secrete HDL- derived progestins despite low levels of SR-BI and barely detectable levels of SR-BII (an isoform of SR-BI). Once stimulated with trophic hormones or Bt2cAMP, small (30-40%) increases are observed in selective pathway functions, but major (~20-fold) increases are seen in SR-BI and SR-BII expression: thus, relatively little is gained in selective cholesteryl ester uptake by mouse granulosa cells even though SR-BI and SR-BII levels are greatly increased. The importance of the HDL receptor proteins to the selective pathway remains clear, however, since a significant portion of the selective process in both basal and stimulated granulosa cells is inhibitable by the use of blocking antibody. Another surface protein, caveolin, previously reported to co-localize with SR-BI in mouse cells shows no change in expression during periods when SR-BI/BII levels are undergoing major shifts.

Original languageEnglish
Pages (from-to)565-576
Number of pages12
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1436
Issue number3
DOIs
StatePublished - Jan 4 1999

Bibliographical note

Funding Information:
This work was supported by grants from the National Institute of Health, HL33881, HL 32868, HL 58012, HL 59376 and the Office of Research and Development, Medical Research Service, Department of Veteran Affairs.

Keywords

  • (Mouse)
  • High density lipoprotein receptor
  • Ovarian granulose cell
  • SR-BI
  • Selective HDL-CE uptake

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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