The semi-automated development of plant cell wall finite element models

Andrew Sayad, Yusuf Oduntan, Norbert Bokros, Seth DeBolt, Alice Benzecry, Daniel J. Robertson, Christopher J. Stubbs

Research output: Contribution to journalArticlepeer-review


This study presents a methodology for a high-throughput digitization and quantification process of plant cell walls characterization, including the automated development of two-dimensional finite element models. Custom algorithms based on machine learning can also analyze the cellular microstructure for phenotypes such as cell size, cell wall curvature, and cell wall orientation. To demonstrate the utility of these models, a series of compound microscope images of both herbaceous and woody representatives were observed and processed. In addition, parametric analyses were performed on the resulting finite element models. Sensitivity analyses of the structural stiffness of the resulting tissue based on the cell wall elastic modulus and the cell wall thickness; demonstrated that the cell wall thickness has a three-fold larger impact of tissue stiffness than cell wall elastic modulus.

Original languageEnglish
Article number3
JournalPlant Methods
Issue number1
StatePublished - Dec 2023

Bibliographical note

Funding Information:
This work was funded in part by the National Science Foundation (Award #1826715) and the United States Department of Agriculture—National Institute of Food and Agriculture (Award #2020-10917). Any opinions, findings, conclusions, or recommendations are those of the author(s) and do not necessarily reflect the view of the funding bodies.

Publisher Copyright:
© 2023, The Author(s).

ASJC Scopus subject areas

  • Biotechnology
  • Genetics
  • Plant Science


Dive into the research topics of 'The semi-automated development of plant cell wall finite element models'. Together they form a unique fingerprint.

Cite this