TY - JOUR
T1 - The soybean Rfg1 gene restricts nodulation by Sinorhizobium fredii USDA193
AU - Fan, Yinglun
AU - Liu, Jinge
AU - Lyu, Shanhua
AU - Wang, Qi
AU - Yang, Shengming
AU - Zhu, Hongyan
N1 - Publisher Copyright:
© 2017 Fan, Liu, Lyu, Wang, Yang and Zhu.
PY - 2017/9/7
Y1 - 2017/9/7
N2 - Sinorhizobium fredii is a fast-growing rhizobial species that can establish a nitrogenfixing symbiosis with a wide range of legume species including soybeans (Glycine max). In soybeans, this interaction shows a high level of specificity such that particular S. fredii strains nodulate only a limited set of plant genotypes. Here we report the identification of a dominant gene in soybeans that restricts nodulation with S. fredii USDA193. Genetic mapping in an F2 population revealed co-segregation of the underlying locus with the previously cloned Rfg1 gene. The Rfg1 allele encodes a member of the Toll-interleukin receptor/nucleotide-binding site/leucine-rich repeat class of plant resistance proteins that restricts nodulation by S. fredii strains USDA257 and USDA205, and an allelic variant of this gene also restricts nodulation by Bradyrhizobium japonicum USDA122. By means of complementation tests and CRISPR/Cas9-mediated gene knockouts, we demonstrate that the Rfg1 allele also is responsible for resistance to nodulation by S. fredii USDA193. Therefore, the Rfg1 allele likely provides broad-spectrum resistance to nodulation by many S. fredii and B. japonicum strains in soybeans.
AB - Sinorhizobium fredii is a fast-growing rhizobial species that can establish a nitrogenfixing symbiosis with a wide range of legume species including soybeans (Glycine max). In soybeans, this interaction shows a high level of specificity such that particular S. fredii strains nodulate only a limited set of plant genotypes. Here we report the identification of a dominant gene in soybeans that restricts nodulation with S. fredii USDA193. Genetic mapping in an F2 population revealed co-segregation of the underlying locus with the previously cloned Rfg1 gene. The Rfg1 allele encodes a member of the Toll-interleukin receptor/nucleotide-binding site/leucine-rich repeat class of plant resistance proteins that restricts nodulation by S. fredii strains USDA257 and USDA205, and an allelic variant of this gene also restricts nodulation by Bradyrhizobium japonicum USDA122. By means of complementation tests and CRISPR/Cas9-mediated gene knockouts, we demonstrate that the Rfg1 allele also is responsible for resistance to nodulation by S. fredii USDA193. Therefore, the Rfg1 allele likely provides broad-spectrum resistance to nodulation by many S. fredii and B. japonicum strains in soybeans.
KW - Nitrogen fixation
KW - Nodulation
KW - Rhizobial symbiosis
KW - Soybean
KW - Symbiosis specificity
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U2 - 10.3389/fpls.2017.01548
DO - 10.3389/fpls.2017.01548
M3 - Article
AN - SCOPUS:85029112335
SN - 1664-462X
VL - 8
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
M1 - 1548
ER -