The stability and biochemical basis of fufenozide resistance in a laboratory-selected strain of Plutella xylostella

Baozhen Tang, Jingyan Sun, Xuguo Zhou, Xiwu Gao, Pei Liang

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Fufenozide, a novel non-steroidal ecdysone agonist, exhibits good efficacy and plays an important role in controlling lepidopteran pests in China. A high level of resistance to fufenozide (320.3-fold) in Plutella xylostella (L.) has been selected under laboratory conditions. In this study, the stability and biochemical basis of resistance to fufenozide were investigated. The resistance ratio to fufezonide declined substantially from 320.3- to 20.5-fold in the fufenozide resistant strain (JSR) when the selection pressure was removed for six consecutive generations. However, the susceptibility to fufezonide could not be fully restored after 28 generations without exposure to the compound. Increased relative fitness (0.87) was associated with the rapid reversal of resistance in the resistance decaying strain (JSRD). The enzymatic analyses showed reduced specific activities of monooxygenase (MO), glutathione S-transferases (GSTs), carboxylesterase and aryl-acylamidase in the JSRD strain, whereas the activity of chitinase increased. The current findings suggest the selected resistance to fufenozide in P. xylostella is not stable, and the four detoxifying enzymes including MO, GSTs, carboxylesterase, and aryl-acylamidase may be involved. For resistance management proposes, insecticide rotation should be a viable option to delay the occurrence of fufenozide resistance in the field.

Original languageEnglish
Pages (from-to)80-85
Number of pages6
JournalPesticide Biochemistry and Physiology
Volume101
Issue number2
DOIs
StatePublished - Oct 2011

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (30971941, 30671389 and 30300232) and Special Fund for Agro-scientific Research in the Public Interest (201103021).

Keywords

  • Enzyme assay
  • Fitness cost
  • Fufenozide
  • Plutella xylostella
  • Resistance-stability

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

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