The SV40 small t intron is accurately and efficiently spliced in tobacco cells

Arthur G. Hunt, Bradley D. Mogen, Nathan M. Chu, Nam Hai Chua

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

We have introduced the SV40 small t intron into tobacco cells as part of a cauliflower mosaic virus 35S promoter-chloramphenicol acetyltransferase-SV40 transcription unit. We find that the small t intron is efficiently and accurately spliced in transgenic tobacco cells that carry this transcription unit. Our results indicate that there is substantial conservation of RNA processing signals between plants and animals, more than has been previously assumed. They also suggest that pre-mRNA processing in plants requires multiple branch sites for efficient processing.

Original languageEnglish
Pages (from-to)375-379
Number of pages5
JournalPlant Molecular Biology
Volume16
Issue number3
DOIs
StatePublished - Mar 1991

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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