The trigeminal sensory system was evaluated for the retrograde transfer of gene therapy vectors into the CNS. The feline immunodeficiency viral vector, FIV(HEXB), encoding for the human HEXB gene, was injected intra-articularly in the temporomandibular joint of 12 week-old HexB-/- mice displaying clinical and histopathological signs of Sandhoff disease. This treatment regiment reduced GM2 storage and ameliorated neuroinflammation in the brain of HexB-/- mice, as well as attenuated behavioral deficits. In conclusion, retrograde transfer along trigeminal sensory nerves may prove to be a valuable route of gene therapy administration for the treatment of lysosomal storage disorders and other neurodegenerative diseases.
|Number of pages||4|
|Journal||Journal of Neuroimmunology|
|State||Published - Apr 30 2009|
Bibliographical noteFunding Information:
This work was funded in part by grant NS048339 from the National Institutes of Health.
- GM gangliosidosis
- Mouse, Gene therapy
- Sandhoff disease
ASJC Scopus subject areas
- Immunology and Allergy
- Clinical Neurology