The Tubulation Activity of a Fission Yeast F-BAR Protein Is Dispensable for Its Function in Cytokinesis

Nathan A. McDonald, Yoshimasa Takizawa, Anna Feoktistova, Ping Xu, Melanie D. Ohi, Craig W. Vander Kooi, Kathleen L. Gould

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


F-BAR proteins link cellular membranes to the actin cytoskeleton in many biological processes. Here we investigated the function of the Schizosaccharomyces pombe Imp2 F-BAR domain in cytokinesis and find that it is critical for Imp2's role in contractile ring constriction and disassembly. To understand mechanistically how the F-BAR domain functions, we determined its structure, elucidated how it interacts with membranes, and identified an interaction between dimers that allows helical oligomerization and membrane tubulation. Using mutations that block either membrane binding or tubulation, we find that membrane binding is required for Imp2's cytokinetic function but that oligomerization and tubulation, activities often deemed central to F-BAR protein function, are dispensable. Accordingly, F-BARs that do not have the capacity to tubulate membranes functionally substitute for the Imp2 F-BAR, establishing that its major role is as a cell-cycle-regulated bridge between the membrane and Imp2 protein partners, rather than as a driver of membrane curvature.

Original languageEnglish
Pages (from-to)534-546
Number of pages13
JournalCell Reports
Issue number3
StatePublished - Jan 26 2016

Bibliographical note

Funding Information:
The authors thank Dr. Rachel Roberts-Galbraith and members of the K.L.G. laboratory, especially Dr. Janel Beckley, for critical review of the manuscript; the Vanderbilt Center for Structural Biology for Analytical Ultracentrifuge and Electron Microscopy Facility support; and NIH grant P20GM103486 for University of Kentucky core support. N.A.M. was supported by AHA fellowship 15PRE21780003. This work was supported by NIH grant GM101035 to K.L.G.

Publisher Copyright:
© 2016 The Authors.

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (all)


Dive into the research topics of 'The Tubulation Activity of a Fission Yeast F-BAR Protein Is Dispensable for Its Function in Cytokinesis'. Together they form a unique fingerprint.

Cite this