The type II secretion system: Biogenesis, molecular architecture and mechanism

Konstantin V. Korotkov; Maria Sandkvist; Wim G.J. Hol

doi:10.1038/nrmicro2762

The type II secretion system: Biogenesis, molecular architecture and mechanism

Konstantin V. Korotkov, Maria Sandkvist, Wim G.J. Hol

Research output: Contribution to journal › Review article › peer-review

395 Scopus citations

Abstract

Many Gram-negative bacteria use the sophisticated type II secretion system (T2SS) to translocate a wide range of proteins from the periplasm across the outer membrane. The inner-membrane platform of the T2SS is the nexus of the system and orchestrates the secretion process through its interactions with the periplasmic filamentous pseudopilus, the dodecameric outer-membrane complex and a cytoplasmic secretion ATPase. Here, recent structural and biochemical information is reviewed to describe our current knowledge of the biogenesis and architecture of the T2SS and its mechanism of action.

Original language	English
Pages (from-to)	336-351
Number of pages	16
Journal	Nature Reviews Microbiology
Volume	10
Issue number	5
DOIs	https://doi.org/10.1038/nrmicro2762
State	Published - May 2012

Bibliographical note

Funding Information:
The authors thank the many members of their groups who have made important contributions to the studies reported, and special thanks go to J. Abendroth, M. Robien, S. Turley, T. Johnson, M. Patrick and M. Gray. They also thank their many collaborators on the T2SS project, including J. Steyaert and E. Pardon for the preparation of valuable nanobodies, and T. Gonen and his group for electron microscopy studies. This work was supported by awards RO1AI049294 (to M.S.) and RO1AI34501 (to W.G.J.H.) from the US National Institutes of Health, National Institute of Allergy and Infectious Diseases, and earlier support from the Howard Hughes Medical Institute to W.G.J.H. is deeply appreciated.

ASJC Scopus subject areas

Microbiology
General Immunology and Microbiology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/nrmicro2762

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title = "The type II secretion system: Biogenesis, molecular architecture and mechanism",

abstract = "Many Gram-negative bacteria use the sophisticated type II secretion system (T2SS) to translocate a wide range of proteins from the periplasm across the outer membrane. The inner-membrane platform of the T2SS is the nexus of the system and orchestrates the secretion process through its interactions with the periplasmic filamentous pseudopilus, the dodecameric outer-membrane complex and a cytoplasmic secretion ATPase. Here, recent structural and biochemical information is reviewed to describe our current knowledge of the biogenesis and architecture of the T2SS and its mechanism of action.",

author = "Korotkov, {Konstantin V.} and Maria Sandkvist and Hol, {Wim G.J.}",

note = "Funding Information: The authors thank the many members of their groups who have made important contributions to the studies reported, and special thanks go to J. Abendroth, M. Robien, S. Turley, T. Johnson, M. Patrick and M. Gray. They also thank their many collaborators on the T2SS project, including J. Steyaert and E. Pardon for the preparation of valuable nanobodies, and T. Gonen and his group for electron microscopy studies. This work was supported by awards RO1AI049294 (to M.S.) and RO1AI34501 (to W.G.J.H.) from the US National Institutes of Health, National Institute of Allergy and Infectious Diseases, and earlier support from the Howard Hughes Medical Institute to W.G.J.H. is deeply appreciated.",

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AU - Sandkvist, Maria

AU - Hol, Wim G.J.

N1 - Funding Information: The authors thank the many members of their groups who have made important contributions to the studies reported, and special thanks go to J. Abendroth, M. Robien, S. Turley, T. Johnson, M. Patrick and M. Gray. They also thank their many collaborators on the T2SS project, including J. Steyaert and E. Pardon for the preparation of valuable nanobodies, and T. Gonen and his group for electron microscopy studies. This work was supported by awards RO1AI049294 (to M.S.) and RO1AI34501 (to W.G.J.H.) from the US National Institutes of Health, National Institute of Allergy and Infectious Diseases, and earlier support from the Howard Hughes Medical Institute to W.G.J.H. is deeply appreciated.

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N2 - Many Gram-negative bacteria use the sophisticated type II secretion system (T2SS) to translocate a wide range of proteins from the periplasm across the outer membrane. The inner-membrane platform of the T2SS is the nexus of the system and orchestrates the secretion process through its interactions with the periplasmic filamentous pseudopilus, the dodecameric outer-membrane complex and a cytoplasmic secretion ATPase. Here, recent structural and biochemical information is reviewed to describe our current knowledge of the biogenesis and architecture of the T2SS and its mechanism of action.

AB - Many Gram-negative bacteria use the sophisticated type II secretion system (T2SS) to translocate a wide range of proteins from the periplasm across the outer membrane. The inner-membrane platform of the T2SS is the nexus of the system and orchestrates the secretion process through its interactions with the periplasmic filamentous pseudopilus, the dodecameric outer-membrane complex and a cytoplasmic secretion ATPase. Here, recent structural and biochemical information is reviewed to describe our current knowledge of the biogenesis and architecture of the T2SS and its mechanism of action.

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JO - Nature Reviews Microbiology

JF - Nature Reviews Microbiology

IS - 5

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The type II secretion system: Biogenesis, molecular architecture and mechanism

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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