The yeast polyadenylate-binding protein (PAB1) gene acts as a disease lesion mimic gene when expressed in plants

Qingshun Li, Carol Von Lanken, Jianjun Yang, Christopher B. Lawrence, Arthur G. Hunt

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


We have expressed the gene (PAB1) encoding the yeast polyadenylate-binding protein (Pab1p) in tobacco. Plants that accumulate the Pab1p display a range of abnormalities, ranging from a characteristic chlorosis in leaves to a necrosis and large inhibition of growth. The severity of these abnormalities reflects the levels of yeast Pab1p expression in the transgenic plants. In contrast, no obvious differences could be seen in callus cultures between the transgene and vector control. Plants that display PAB-associated abnormalities were resistant to a range of plant pathogens, and had elevated levels of expression of a pathogenesis-related gene. These two properties - impairment of growth and induction of defense responses - indicate that the yeast PAB1 gene can act as a disease lesion mimic gene in plants.

Original languageEnglish
Pages (from-to)335-344
Number of pages10
JournalPlant Molecular Biology
Issue number2
StatePublished - 2000

Bibliographical note

Funding Information:
We are grateful to Maurice Swanson for the gift of antibody against the yeast PAB and Robert Houtz for providing anti-rbcL antisera. This work was supported in part by USDA-University of Kentucky Cooperative Agreement 58-43YK-7-0025 and USDA Competitive Grant 89-37262-4835. Q.L. and C.L. were supported by funds from the Kentucky Tobacco Research Board.


  • Disease resistance
  • Lesion mimic
  • Poly(A)-binding proteins
  • Posttranscriptional control
  • SAR

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science


Dive into the research topics of 'The yeast polyadenylate-binding protein (PAB1) gene acts as a disease lesion mimic gene when expressed in plants'. Together they form a unique fingerprint.

Cite this