Thermally responsive membrane-based microbiological sensing component for early detection of membrane biofouling

Guang Cai, Colleen Gorey, Amr Zaky, Isabel Escobar, Cyndee Gruden

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Early detection of bacteria in water treatment is desired to mitigate system biofouling and potential human health impacts. This research involved the development of an antibody-based sensor, which was attached to a hydroxypropyl cellulose (HPC) modified cellulose acetate ultrafiltration membrane surface, to target bacteria. HPC was chosen because it collapses above and expands below a lower critical solution temperature (43. °C), thus allowing temperature modulation. The membrane had a high target recovery efficiency (10-18%) in both single bacterium and complex samples (simulated by adding organics and competitive organisms). Sensor recovery was decreased (to 5-7%) following temperature activation (above 50. °C) due to adverse impacts of temperature on the antibody. The membrane was able to be regenerated (with 0.1. M NaOH) and reused three consecutive times. The thermally responsive sensing membrane developed from this work was specifically developed to detect biofoulants in membrane-based water treatment processes. However, it could be adapted to address a range of environmental detection concerns, such as pathogen detection.

Original languageEnglish
Pages (from-to)116-123
Number of pages8
JournalDesalination
Volume270
Issue number1-3
DOIs
StatePublished - Apr 1 2011

Bibliographical note

Funding Information:
We are grateful to DARPA (Grant HR0011-07-1-0003 ) and NSF (CBET 0610624 ) for funding this research. We also wish to express our sincere thanks to Dr Steven Kloos and Ms Jessica Schloss of GE water and Process Technologies for providing membranes.

Keywords

  • Antibody
  • Detection
  • Membrane
  • Sensor

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering
  • General Materials Science
  • Water Science and Technology
  • Mechanical Engineering

Fingerprint

Dive into the research topics of 'Thermally responsive membrane-based microbiological sensing component for early detection of membrane biofouling'. Together they form a unique fingerprint.

Cite this