Abstract
Early detection of bacteria in water treatment is desired to mitigate system biofouling and potential human health impacts. This research involved the development of an antibody-based sensor, which was attached to a hydroxypropyl cellulose (HPC) modified cellulose acetate ultrafiltration membrane surface, to target bacteria. HPC was chosen because it collapses above and expands below a lower critical solution temperature (43. °C), thus allowing temperature modulation. The membrane had a high target recovery efficiency (10-18%) in both single bacterium and complex samples (simulated by adding organics and competitive organisms). Sensor recovery was decreased (to 5-7%) following temperature activation (above 50. °C) due to adverse impacts of temperature on the antibody. The membrane was able to be regenerated (with 0.1. M NaOH) and reused three consecutive times. The thermally responsive sensing membrane developed from this work was specifically developed to detect biofoulants in membrane-based water treatment processes. However, it could be adapted to address a range of environmental detection concerns, such as pathogen detection.
| Original language | English |
|---|---|
| Pages (from-to) | 116-123 |
| Number of pages | 8 |
| Journal | Desalination |
| Volume | 270 |
| Issue number | 1-3 |
| DOIs | |
| State | Published - Apr 1 2011 |
Bibliographical note
Funding Information:We are grateful to DARPA (Grant HR0011-07-1-0003 ) and NSF (CBET 0610624 ) for funding this research. We also wish to express our sincere thanks to Dr Steven Kloos and Ms Jessica Schloss of GE water and Process Technologies for providing membranes.
Keywords
- Antibody
- Detection
- Membrane
- Sensor
ASJC Scopus subject areas
- General Chemistry
- General Chemical Engineering
- General Materials Science
- Water Science and Technology
- Mechanical Engineering
