Abstract
Positive-strand RNA viruses, which can be devastating pathogens in humans, animals and plants, replicate their genomes on intracellular membranes. Here, we describe the three-dimensional ultrastructural organization of a tombusvirus replicase in yeast, a valuable model for exploring virus-host interactions. We visualized the intracellular distribution of a viral replicase protein using metal-tagging transmission electron microscopy, a highly sensitive nanotechnology whose full potential remains to be developed. These three-dimensional images show how viral replicase molecules are organized when they are incorporated into the active domains of the intracellular replication compartment. Our approach provides a means to study protein activation mechanisms in cells and to identify targets for new antiviral compounds.
Original language | English |
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Pages (from-to) | 260-268 |
Number of pages | 9 |
Journal | Journal of Cell Science |
Volume | 130 |
Issue number | 1 |
DOIs | |
State | Published - 2017 |
Bibliographical note
Publisher Copyright:© 2017. Published by The Company of Biologists Ltd.
Keywords
- 3D electron microscopy
- Clonable tag
- Metal-tagging transmission electron microscopy
- Tomography
- Viral replication factory
ASJC Scopus subject areas
- Cell Biology