Three levels of simple morphophysiological dormancy in seeds of Ilex (Aquifoliaceae) species from Argentina

Guadalupe Galíndez, Diana Ceccato, Rosana Bubillo, Lucía Lindow-López, Gisela Malagrina, Pablo Ortega-Baes, Carol C. Baskin

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

As a contribution to understanding the world biogeography of seed dormancy in the cosmopolitan genus Ilex, we studied seeds of I. Argentina, I. brasiliensis, I. brevicuspis, I. dumosa, I. paraguariensis and I. theezans from the subtropical region of Argentina. We hypothesized that seeds of these species have non-deep simple morphophysiological dormancy (MPD). Effects of temperature, cold stratification and gibberellic acid (GA3) on seed germination and embryo growth were tested. Regardless of incubation temperature, little or no germination occurred for any species until ≥6 weeks. There was an up to 3-fold increase in embryo length to seed length (E:S) ratio before seeds germinated, and embryos grew only during warm-stratifying conditions. Seeds of I. brasiliensis, I. brevicuspis and I. theezans had non-deep simple MPD and germinated to ≥80% after 12, 24 and 16 weeks, respectively. Cold stratification increased germination of I. brasiliensis and I. brevicuspis, and GA3 increased the rate but not final germination percentage of I. brasiliensis and I. theezans. Fresh seeds of I. dumosa required 40 weeks of warm stratification to germinate to 53%, while those after-ripened for 2 months germinated to 81% after 30 weeks; this species has intermediate simple MPD. Seeds of I. Argentina and I. paraguariensis germinated to 15 and 21%, respectively, after 40 weeks of warm stratification and did not after-ripen or respond to GA3; these seeds have deep simple MPD. This is the first report of intermediate and deep simple MPD that is broken by warm stratification, thereby increasing our knowledge of seed dormancy in Ilex and in subtropical regions.

Original languageEnglish
Pages (from-to)131-139
Number of pages9
JournalSeed Science Research
Volume28
Issue number2
DOIs
StatePublished - Jun 1 2018

Bibliographical note

Publisher Copyright:
© 2018 Cambridge University Press.

Keywords

  • Ilex species
  • cold stratification
  • embryo length
  • morphophysiological dormancy
  • move-along experiment
  • seed length ratio
  • underdeveloped embryo
  • warm stratification

ASJC Scopus subject areas

  • Plant Science

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