Thymine DNA glycosylase is a positive regulator of Wnt signaling in colorectal cancer

Xuehe Xu; Tianxin Yu; Jiandang Shi; Xi Chen; Wen Zhang; Ting Lin; Zhihong Liu; Yadong Wang; Zheng Zeng; Chi Wang; Mingsong Li; Chunming Liu

doi:10.1074/jbc.M113.538835

Thymine DNA glycosylase is a positive regulator of Wnt signaling in colorectal cancer

Xuehe Xu, Tianxin Yu, Jiandang Shi, Xi Chen, Wen Zhang, Ting Lin, Zhihong Liu, Yadong Wang, Zheng Zeng, Chi Wang, Mingsong Li, Chunming Liu

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Wnt signaling plays an important role in colorectal cancer (CRC). Although the mechanisms of β-catenin degradation have been well studied, the mechanism by which β-catenin activates transcription is still not fully understood. While screening a panel of DNA demethylases, we found that thymine DNA glycosylase (TDG) up-regulated Wnt signaling.TDGinteracts with the transcription factor TCF4 and coactivator CREB-binding protein/p300 in the Wnt pathway. Knocking down TDG by shRNAs inhibited the proliferation of CRC cells in vitro and in vivo. In CRC patients, TDG levels were significantly higher in tumor tissues than in the adjacent normal tissues. These results suggest that TDG warrants consideration as a potential biomarker for CRC and as a target for CRC treatment.

Original language	English
Pages (from-to)	8881-8890
Number of pages	10
Journal	Journal of Biological Chemistry
Volume	289
Issue number	13
DOIs	https://doi.org/10.1074/jbc.M113.538835
State	Published - Mar 30 2014

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1074/jbc.M113.538835

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title = "Thymine DNA glycosylase is a positive regulator of Wnt signaling in colorectal cancer",

abstract = "Wnt signaling plays an important role in colorectal cancer (CRC). Although the mechanisms of β-catenin degradation have been well studied, the mechanism by which β-catenin activates transcription is still not fully understood. While screening a panel of DNA demethylases, we found that thymine DNA glycosylase (TDG) up-regulated Wnt signaling.TDGinteracts with the transcription factor TCF4 and coactivator CREB-binding protein/p300 in the Wnt pathway. Knocking down TDG by shRNAs inhibited the proliferation of CRC cells in vitro and in vivo. In CRC patients, TDG levels were significantly higher in tumor tissues than in the adjacent normal tissues. These results suggest that TDG warrants consideration as a potential biomarker for CRC and as a target for CRC treatment.",

author = "Xuehe Xu and Tianxin Yu and Jiandang Shi and Xi Chen and Wen Zhang and Ting Lin and Zhihong Liu and Yadong Wang and Zheng Zeng and Chi Wang and Mingsong Li and Chunming Liu",

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T1 - Thymine DNA glycosylase is a positive regulator of Wnt signaling in colorectal cancer

AU - Xu, Xuehe

AU - Yu, Tianxin

AU - Shi, Jiandang

AU - Chen, Xi

AU - Zhang, Wen

AU - Lin, Ting

AU - Liu, Zhihong

AU - Wang, Yadong

AU - Zeng, Zheng

AU - Wang, Chi

AU - Li, Mingsong

AU - Liu, Chunming

PY - 2014/3/30

Y1 - 2014/3/30

N2 - Wnt signaling plays an important role in colorectal cancer (CRC). Although the mechanisms of β-catenin degradation have been well studied, the mechanism by which β-catenin activates transcription is still not fully understood. While screening a panel of DNA demethylases, we found that thymine DNA glycosylase (TDG) up-regulated Wnt signaling.TDGinteracts with the transcription factor TCF4 and coactivator CREB-binding protein/p300 in the Wnt pathway. Knocking down TDG by shRNAs inhibited the proliferation of CRC cells in vitro and in vivo. In CRC patients, TDG levels were significantly higher in tumor tissues than in the adjacent normal tissues. These results suggest that TDG warrants consideration as a potential biomarker for CRC and as a target for CRC treatment.

AB - Wnt signaling plays an important role in colorectal cancer (CRC). Although the mechanisms of β-catenin degradation have been well studied, the mechanism by which β-catenin activates transcription is still not fully understood. While screening a panel of DNA demethylases, we found that thymine DNA glycosylase (TDG) up-regulated Wnt signaling.TDGinteracts with the transcription factor TCF4 and coactivator CREB-binding protein/p300 in the Wnt pathway. Knocking down TDG by shRNAs inhibited the proliferation of CRC cells in vitro and in vivo. In CRC patients, TDG levels were significantly higher in tumor tissues than in the adjacent normal tissues. These results suggest that TDG warrants consideration as a potential biomarker for CRC and as a target for CRC treatment.

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Thymine DNA glycosylase is a positive regulator of Wnt signaling in colorectal cancer

Abstract

ASJC Scopus subject areas

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