Tissue-specific promoters regulate aromatase cytochrome P450 expression

Evan R. Simpson, Mala S. Mahendroo, Gary D. Means, Michael W. Kilgore, C. Jo Corbin, Carole R. Mendelson

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


In humans, estrogen biosynthesis occurs in several tissue sites, including ovary, placenta, adipose, and brain. Recent work from our laboratory indicates that tissue-specific expression of aromatase cytochrome P450 (P450arom), the enzyme responsible for estrogen biosynthesis, is determined, in part, by the use of tissue-specific promoters. Thus, the expression of P450arom in human ovary appears to utilize a promoter proximal to the translation start site. This promoter is not utilized in placenta; instead, the promoter used to drive aromatase expression in placenta is ≥40 kb upstream from the translational start site. In addition, a minor promoter used in the expression of a small proportion of placental transcripts is 9 kb upstream from the start of translation. Transcripts from these promoters are also expressed in other fetal tissues, including placenta-related cells such as JEG-3 choriocarcinoma cells and hydatidiform moles and other fetal tissues such as fetal liver. In adipose tissue, on the other hand, expression of P450arom may be achieved by yet another, adipose-specific promoter. The various 5′-untranslated exons unique for expression driven by each of these promoters are spliced into a common intron/exon boundary upstream from the translational start site. This means that the protein expressed in each of the various tissue-specific sites of estrogen biosynthesis is identical.

Original languageEnglish
Pages (from-to)317-324
Number of pages8
JournalClinical Chemistry
Issue number2
StatePublished - 1993


  • Adipose tissue
  • Brain
  • Estrogen biosynthesis
  • Genes
  • Ovary
  • Placenta

ASJC Scopus subject areas

  • Medicine (all)


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