Titanium dioxide nanotubes applied to conventional glass ionomer cement influence the expression of immunoinflammatory markers: An in vitro study

João Pedro Rangel-Coelho; Pedro Viel Gogolla; Maria Davoli Meyer; Lucas Carvalho Simão; Bruna Carolina Costa; Renato Côrrea Viana Casarin; Mauro Pedrine Santamaria; Lucas Novaes Teixeira; Daiane Cristina Peruzzo; Paulo Noronha Lisboa-Filho; Francisco Humberto Nociti-Jr; Kamila Rosamilia Kantovitz

doi:10.1016/j.heliyon.2024.e30834

Titanium dioxide nanotubes applied to conventional glass ionomer cement influence the expression of immunoinflammatory markers: An in vitro study

João Pedro Rangel-Coelho
, Pedro Viel Gogolla
, Maria Davoli Meyer
, Lucas Carvalho Simão
, Bruna Carolina Costa
, Renato Côrrea Viana Casarin
, Mauro Pedrine Santamaria
, Lucas Novaes Teixeira
, Daiane Cristina Peruzzo
, Paulo Noronha Lisboa-Filho
, Francisco Humberto Nociti-Jr
, Kamila Rosamilia Kantovitz

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Objectives: To assess the impact of different concentrations TiO₂-nt incorporated into a glass ionomer cement on the proliferation, mitochondrial metabolism, morphology, and pro- and anti-inflammatory cytokine production of cultured fibroblasts (NIH/3T3), whether or not stimulated by lipopolysaccharides (LPS-2 μg/mL, 24 h). Methods: TiO₂-nt was added to KM (Ketac Molar EasyMix™, 3 %, 5 %, 7 % in weight); unblended KM was used as the control. The analyses included: Cell proliferation assay (n = 6; 24/48/72h); Mitochondrial metabolism assay (n = 6; 24/48/72h); Confocal laser microscopy (n = 3; 24/48/72h); Determination of biomarkers (IL-1β/IL-6/IL-10/VEGF/TNF) by using both multiplex technology (n = 6; 12/18 h) and the quantitative real-time PCR assay (q-PCR) (n = 3, 24/72/120 h). The data underwent analysis using both the Shapiro-Wilk and Levene tests, and by generalized linear models (α = 0.05). Results: It demonstrated that cell proliferation increased over time, regardless of the presence of TiO₂-nt or LPS, and displayed a significant increase at 72 h; mitochondrial metabolism increased (p < 0.05), irrespective of exposure to LPS (p = 0.937); no cell morphology changes were observed; TiO₂-nt reverted the impact of KM on the secreted levels of the evaluated proteins and the gene expressions in the presence of LPS (p < 0.0001). Conclusions: TiO₂-nt did not adversely affect the biological behavior of fibroblastic cells cultured on GIC discs.

Original language	English
Article number	e30834
Journal	Heliyon
Volume	10
Issue number	10
DOIs	https://doi.org/10.1016/j.heliyon.2024.e30834
State	Published - May 30 2024

Bibliographical note

Publisher Copyright:
© 2024 The Authors

Funding

The authors extend their gratitude to technicians at the Pathology and Periodontology Labs - São Leopoldo Mandic and Piracicaba Dental School, University of Campinas (UNICAMP), especially Ms. Pollyanna T. Montaldi Pavani and Ms. Fabiana C. Vieira, for their invaluable assistance. Special thanks to Ms. Cristina Martorana for her assistance with text editing. This research received support from the FAPESP (#2019/14078-8, #2019/07363-8, and #2022/04218-0). The authors declare no affiliations with any company related to this research. The authors extend their gratitude to technicians at the Pathology and Periodontology Labs - São Leopoldo Mandic and Piracicaba Dental School, University of Campinas (UNICAMP), especially Ms. Pollyanna T. Montaldi Pavani and Ms. Fabiana C. Vieira, for their invaluable assistance. Special thanks to Ms. Cristina Martorana for her assistance with text editing. This research received support from the FAPESP (#2019/14078-8, #2019/07363-8, #2022/04218-0, and #2024/05610-6). The authors declare no affiliations with any company related to this research.

Funders	Funder number
Fundação de Amparo à Pesquisa do Estado de São Paulo	2019/14078-8, 2022/04218-0, 2019/07363-8, 2024/05610-6
Fundação de Amparo à Pesquisa do Estado de São Paulo

Keywords

Fibroblasts
Glass ionomer cements
Interleukins
Nanotechnology
Proteome
Titanium dioxide

ASJC Scopus subject areas

General

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10.1016/j.heliyon.2024.e30834

Cite this

Rangel-Coelho, J. P., Gogolla, P. V., Meyer, M. D., Simão, L. C., Costa, B. C., Casarin, R. C. V., Santamaria, M. P., Teixeira, L. N., Peruzzo, D. C., Lisboa-Filho, P. N., Nociti-Jr, F. H., & Kantovitz, K. R. (2024). Titanium dioxide nanotubes applied to conventional glass ionomer cement influence the expression of immunoinflammatory markers: An in vitro study. Heliyon, 10(10), Article e30834. https://doi.org/10.1016/j.heliyon.2024.e30834

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title = "Titanium dioxide nanotubes applied to conventional glass ionomer cement influence the expression of immunoinflammatory markers: An in vitro study",

abstract = "Objectives: To assess the impact of different concentrations TiO2-nt incorporated into a glass ionomer cement on the proliferation, mitochondrial metabolism, morphology, and pro- and anti-inflammatory cytokine production of cultured fibroblasts (NIH/3T3), whether or not stimulated by lipopolysaccharides (LPS-2 μg/mL, 24 h). Methods: TiO2-nt was added to KM (Ketac Molar EasyMix{\texttrademark}, 3 \%, 5 \%, 7 \% in weight); unblended KM was used as the control. The analyses included: Cell proliferation assay (n = 6; 24/48/72h); Mitochondrial metabolism assay (n = 6; 24/48/72h); Confocal laser microscopy (n = 3; 24/48/72h); Determination of biomarkers (IL-1β/IL-6/IL-10/VEGF/TNF) by using both multiplex technology (n = 6; 12/18 h) and the quantitative real-time PCR assay (q-PCR) (n = 3, 24/72/120 h). The data underwent analysis using both the Shapiro-Wilk and Levene tests, and by generalized linear models (α = 0.05). Results: It demonstrated that cell proliferation increased over time, regardless of the presence of TiO2-nt or LPS, and displayed a significant increase at 72 h; mitochondrial metabolism increased (p < 0.05), irrespective of exposure to LPS (p = 0.937); no cell morphology changes were observed; TiO2-nt reverted the impact of KM on the secreted levels of the evaluated proteins and the gene expressions in the presence of LPS (p < 0.0001). Conclusions: TiO2-nt did not adversely affect the biological behavior of fibroblastic cells cultured on GIC discs.",

keywords = "Fibroblasts, Glass ionomer cements, Interleukins, Nanotechnology, Proteome, Titanium dioxide",

author = "Rangel-Coelho, \{Jo{\~a}o Pedro\} and Gogolla, \{Pedro Viel\} and Meyer, \{Maria Davoli\} and Sim{\~a}o, \{Lucas Carvalho\} and Costa, \{Bruna Carolina\} and Casarin, \{Renato C{\^o}rrea Viana\} and Santamaria, \{Mauro Pedrine\} and Teixeira, \{Lucas Novaes\} and Peruzzo, \{Daiane Cristina\} and Lisboa-Filho, \{Paulo Noronha\} and Nociti-Jr, \{Francisco Humberto\} and Kantovitz, \{Kamila Rosamilia\}",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors",

year = "2024",

month = may,

day = "30",

doi = "10.1016/j.heliyon.2024.e30834",

language = "English",

volume = "10",

number = "10",

}

Rangel-Coelho, JP, Gogolla, PV, Meyer, MD, Simão, LC, Costa, BC, Casarin, RCV, Santamaria, MP, Teixeira, LN, Peruzzo, DC, Lisboa-Filho, PN, Nociti-Jr, FH & Kantovitz, KR 2024, 'Titanium dioxide nanotubes applied to conventional glass ionomer cement influence the expression of immunoinflammatory markers: An in vitro study', Heliyon, vol. 10, no. 10, e30834. https://doi.org/10.1016/j.heliyon.2024.e30834

TY - JOUR

T1 - Titanium dioxide nanotubes applied to conventional glass ionomer cement influence the expression of immunoinflammatory markers

T2 - An in vitro study

AU - Rangel-Coelho, João Pedro

AU - Gogolla, Pedro Viel

AU - Meyer, Maria Davoli

AU - Simão, Lucas Carvalho

AU - Costa, Bruna Carolina

AU - Casarin, Renato Côrrea Viana

AU - Santamaria, Mauro Pedrine

AU - Teixeira, Lucas Novaes

AU - Peruzzo, Daiane Cristina

AU - Lisboa-Filho, Paulo Noronha

AU - Nociti-Jr, Francisco Humberto

AU - Kantovitz, Kamila Rosamilia

PY - 2024/5/30

Y1 - 2024/5/30

N2 - Objectives: To assess the impact of different concentrations TiO2-nt incorporated into a glass ionomer cement on the proliferation, mitochondrial metabolism, morphology, and pro- and anti-inflammatory cytokine production of cultured fibroblasts (NIH/3T3), whether or not stimulated by lipopolysaccharides (LPS-2 μg/mL, 24 h). Methods: TiO2-nt was added to KM (Ketac Molar EasyMix™, 3 %, 5 %, 7 % in weight); unblended KM was used as the control. The analyses included: Cell proliferation assay (n = 6; 24/48/72h); Mitochondrial metabolism assay (n = 6; 24/48/72h); Confocal laser microscopy (n = 3; 24/48/72h); Determination of biomarkers (IL-1β/IL-6/IL-10/VEGF/TNF) by using both multiplex technology (n = 6; 12/18 h) and the quantitative real-time PCR assay (q-PCR) (n = 3, 24/72/120 h). The data underwent analysis using both the Shapiro-Wilk and Levene tests, and by generalized linear models (α = 0.05). Results: It demonstrated that cell proliferation increased over time, regardless of the presence of TiO2-nt or LPS, and displayed a significant increase at 72 h; mitochondrial metabolism increased (p < 0.05), irrespective of exposure to LPS (p = 0.937); no cell morphology changes were observed; TiO2-nt reverted the impact of KM on the secreted levels of the evaluated proteins and the gene expressions in the presence of LPS (p < 0.0001). Conclusions: TiO2-nt did not adversely affect the biological behavior of fibroblastic cells cultured on GIC discs.

AB - Objectives: To assess the impact of different concentrations TiO2-nt incorporated into a glass ionomer cement on the proliferation, mitochondrial metabolism, morphology, and pro- and anti-inflammatory cytokine production of cultured fibroblasts (NIH/3T3), whether or not stimulated by lipopolysaccharides (LPS-2 μg/mL, 24 h). Methods: TiO2-nt was added to KM (Ketac Molar EasyMix™, 3 %, 5 %, 7 % in weight); unblended KM was used as the control. The analyses included: Cell proliferation assay (n = 6; 24/48/72h); Mitochondrial metabolism assay (n = 6; 24/48/72h); Confocal laser microscopy (n = 3; 24/48/72h); Determination of biomarkers (IL-1β/IL-6/IL-10/VEGF/TNF) by using both multiplex technology (n = 6; 12/18 h) and the quantitative real-time PCR assay (q-PCR) (n = 3, 24/72/120 h). The data underwent analysis using both the Shapiro-Wilk and Levene tests, and by generalized linear models (α = 0.05). Results: It demonstrated that cell proliferation increased over time, regardless of the presence of TiO2-nt or LPS, and displayed a significant increase at 72 h; mitochondrial metabolism increased (p < 0.05), irrespective of exposure to LPS (p = 0.937); no cell morphology changes were observed; TiO2-nt reverted the impact of KM on the secreted levels of the evaluated proteins and the gene expressions in the presence of LPS (p < 0.0001). Conclusions: TiO2-nt did not adversely affect the biological behavior of fibroblastic cells cultured on GIC discs.

KW - Fibroblasts

KW - Glass ionomer cements

KW - Interleukins

KW - Nanotechnology

KW - Proteome

KW - Titanium dioxide

UR - https://www.scopus.com/pages/publications/85192833826

UR - https://www.scopus.com/pages/publications/85192833826#tab=citedBy

U2 - 10.1016/j.heliyon.2024.e30834

DO - 10.1016/j.heliyon.2024.e30834

M3 - Article

AN - SCOPUS:85192833826

SN - 2405-8440

VL - 10

JO - Heliyon

JF - Heliyon

IS - 10

M1 - e30834

ER -

Titanium dioxide nanotubes applied to conventional glass ionomer cement influence the expression of immunoinflammatory markers: An in vitro study

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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