Abstract
Radiation injury to the central nervous system (CNS) results in glial activation accompanied by expression of pro-inflammatory cytokines and adhesion molecules. In this study we demonstrate intercellular adhesion molecule-1 (ICAM-1) induction in the irradiated mouse brain at the mRNA and protein levels. Immunocytochemical analysis revealed that ICAM-1 protein was primarily expressed in endothelial cells and microglia. In vitro, ionizing radiation significantly induces TNFα, IL-1β and ICAM-1 mRNA in primary microglia cultures. Interestingly, although ionizing radiation activated primary astrocyte cultures, it did not induce ICAM-1 expression. However, exposure of astrocytes to conditioned medium collected from irradiated microglia resulted in ICAM-1 induction, which was abrogated when the conditioned medium was pre-incubated with neutralizing antibodies raised against murine TNFα and IL-1β. These results indicate that pro- inflammatory cytokines may be necessary for ICAM-1 expression in astrocytes in CNS radiation injury.
Original language | English |
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Pages (from-to) | 95-106 |
Number of pages | 12 |
Journal | Journal of Neuroimmunology |
Volume | 95 |
Issue number | 1-2 |
DOIs | |
State | Published - Mar 1 1999 |
Bibliographical note
Funding Information:The authors thank Eric Hernady for his assistance in irradiating cell cultures. This work was supported by PHS grants CA11051 and NS 33553 from the National Institutes of Health.
Keywords
- Astrocytes
- Brain
- Intercellular adhesion molecule-1
- Ionizing radiation
- Microglia
- Mouse
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Neurology
- Clinical Neurology