TNFα and IL-1β mediate intercellular adhesion molecule-1 induction via microglia-astrocyte interaction in CNS radiation injury

Stephanos Kyrkanides, John A. Olschowka, Jacqueline P. Williams, John T. Hansen, M. Kerry O'Banion

Research output: Contribution to journalArticlepeer-review

150 Scopus citations

Abstract

Radiation injury to the central nervous system (CNS) results in glial activation accompanied by expression of pro-inflammatory cytokines and adhesion molecules. In this study we demonstrate intercellular adhesion molecule-1 (ICAM-1) induction in the irradiated mouse brain at the mRNA and protein levels. Immunocytochemical analysis revealed that ICAM-1 protein was primarily expressed in endothelial cells and microglia. In vitro, ionizing radiation significantly induces TNFα, IL-1β and ICAM-1 mRNA in primary microglia cultures. Interestingly, although ionizing radiation activated primary astrocyte cultures, it did not induce ICAM-1 expression. However, exposure of astrocytes to conditioned medium collected from irradiated microglia resulted in ICAM-1 induction, which was abrogated when the conditioned medium was pre-incubated with neutralizing antibodies raised against murine TNFα and IL-1β. These results indicate that pro- inflammatory cytokines may be necessary for ICAM-1 expression in astrocytes in CNS radiation injury.

Original languageEnglish
Pages (from-to)95-106
Number of pages12
JournalJournal of Neuroimmunology
Volume95
Issue number1-2
DOIs
StatePublished - Mar 1 1999

Bibliographical note

Funding Information:
The authors thank Eric Hernady for his assistance in irradiating cell cultures. This work was supported by PHS grants CA11051 and NS 33553 from the National Institutes of Health.

Keywords

  • Astrocytes
  • Brain
  • Intercellular adhesion molecule-1
  • Ionizing radiation
  • Microglia
  • Mouse

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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