TNF induces cytoplasmic vesicles in actinomycin D-treated K 562 cells

R. Munker; L. Greither; M. Darsow; S. Pander; W. Wilmanns

doi:10.1007/BF01715127

TNF induces cytoplasmic vesicles in actinomycin D-treated K 562 cells

R. Munker
, L. Greither
, M. Darsow
, S. Pander
, W. Wilmanns

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We report here that tumor necrosis factor alpha (TNF) induces peculiar cytoplasmic vesicles in the human erythromyeloid leukemia cell line K 562, sensitized to the cytotoxic action of TNF by a treatment with the inhibitor of transcription actinomycin D. These vesicles are well delineated ultrastructurally. The formation of these vesicles is characteristic for the combination of actinomycin D with TNF and precedes the changes of apoptosis and cellular disintegration. These vesicles correspond to an intermediate step in the cytotoxicity caused by TNF and may indicate that reactive metabolites are involved in the mechanism of action of TNF.

Original language	English
Pages (from-to)	50-52
Number of pages	3
Journal	Annals of Hematology
Volume	65
Issue number	1
DOIs	https://doi.org/10.1007/BF01715127
State	Published - Jul 1992

Keywords

K 562
Leukemia
TNF
Ultrastructure

ASJC Scopus subject areas

Hematology

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10.1007/BF01715127

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title = "TNF induces cytoplasmic vesicles in actinomycin D-treated K 562 cells",

abstract = "We report here that tumor necrosis factor alpha (TNF) induces peculiar cytoplasmic vesicles in the human erythromyeloid leukemia cell line K 562, sensitized to the cytotoxic action of TNF by a treatment with the inhibitor of transcription actinomycin D. These vesicles are well delineated ultrastructurally. The formation of these vesicles is characteristic for the combination of actinomycin D with TNF and precedes the changes of apoptosis and cellular disintegration. These vesicles correspond to an intermediate step in the cytotoxicity caused by TNF and may indicate that reactive metabolites are involved in the mechanism of action of TNF.",

keywords = "K 562, Leukemia, TNF, Ultrastructure",

author = "R. Munker and L. Greither and M. Darsow and S. Pander and W. Wilmanns",

year = "1992",

month = jul,

doi = "10.1007/BF01715127",

language = "English",

volume = "65",

pages = "50--52",

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TY - JOUR

T1 - TNF induces cytoplasmic vesicles in actinomycin D-treated K 562 cells

AU - Munker, R.

AU - Greither, L.

AU - Darsow, M.

AU - Pander, S.

AU - Wilmanns, W.

PY - 1992/7

Y1 - 1992/7

N2 - We report here that tumor necrosis factor alpha (TNF) induces peculiar cytoplasmic vesicles in the human erythromyeloid leukemia cell line K 562, sensitized to the cytotoxic action of TNF by a treatment with the inhibitor of transcription actinomycin D. These vesicles are well delineated ultrastructurally. The formation of these vesicles is characteristic for the combination of actinomycin D with TNF and precedes the changes of apoptosis and cellular disintegration. These vesicles correspond to an intermediate step in the cytotoxicity caused by TNF and may indicate that reactive metabolites are involved in the mechanism of action of TNF.

AB - We report here that tumor necrosis factor alpha (TNF) induces peculiar cytoplasmic vesicles in the human erythromyeloid leukemia cell line K 562, sensitized to the cytotoxic action of TNF by a treatment with the inhibitor of transcription actinomycin D. These vesicles are well delineated ultrastructurally. The formation of these vesicles is characteristic for the combination of actinomycin D with TNF and precedes the changes of apoptosis and cellular disintegration. These vesicles correspond to an intermediate step in the cytotoxicity caused by TNF and may indicate that reactive metabolites are involved in the mechanism of action of TNF.

KW - K 562

KW - Leukemia

KW - TNF

KW - Ultrastructure

UR - https://www.scopus.com/pages/publications/0026753103

UR - https://www.scopus.com/pages/publications/0026753103#tab=citedBy

U2 - 10.1007/BF01715127

DO - 10.1007/BF01715127

M3 - Article

C2 - 1643161

AN - SCOPUS:0026753103

SN - 0939-5555

VL - 65

SP - 50

EP - 52

JO - Annals of Hematology

JF - Annals of Hematology

IS - 1

ER -

TNF induces cytoplasmic vesicles in actinomycin D-treated K 562 cells

Abstract

Keywords

ASJC Scopus subject areas

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