To assemble or not to assemble: The changing rules of pneumovirus transmission

Nicolás Cifuentes-Muñoz, Rebecca Ellis Dutch

Research output: Contribution to journalReview articlepeer-review

13 Scopus citations

Abstract

Pneumoviruses represent a major public health burden across the world. Respiratory syncytial virus (RSV) and human metapneumovirus (HMPV), two of the most recognizable pediatric infectious agents, belong to this family. These viruses are enveloped with a non-segmented negative-sense RNA genome, and their replication occurs in specialized cytosolic organelles named inclusion bodies (IB). The critical role of IBs in replication of pneumoviruses has begun to be elucidated, and our current understanding suggests they are highly dynamic structures. From IBs, newly synthesized nucleocapsids are transported to assembly sites, potentially via the actin cytoskeleton, to be incorporated into nascent virions. Released virions, which generally contain one genome, can then diffuse in the extracellular environment to target new cells and reinitiate the process of infection. This is a challenging business for virions, which must face several risks including the extracellular immune responses. In addition, several recent studies suggest that successful infection may be achieved more rapidly by multiple, rather than single, genomic copies being deposited into a target cell. Interestingly, recent data indicate that pneumoviruses have several mechanisms that permit their transmission en bloc, i.e. transmission of multiple genomes at the same time. These mechanisms include the well-studied syncytia formation as well as the newly described formation of long actin-based intercellular extensions. These not only permit en bloc viral transmission, but also bypass assembly of complete virions. In this review we describe several aspects of en bloc viral transmission and how these mechanisms are reshaping our understanding of pneumovirus replication, assembly and spread.

Original languageEnglish
Pages (from-to)68-73
Number of pages6
JournalVirus Research
Volume265
DOIs
StatePublished - May 2019

Bibliographical note

Publisher Copyright:
© 2019 Elsevier B.V.

Keywords

  • En bloc
  • Inclusion bodies
  • Nucleocapsids
  • Pneumoviruses
  • Spread

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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