Tomato chlorosis virus infection facilitates bemisia tabaci med reproduction by elevating vitellogenin expression

Liping Huang; Xiaobin Shi; Jizhe Shi; Zhuo Zhang; Yong Fang; Zhanhong Zhang; Qiuyi Pan; Limin Zheng; Yang Gao; Deyong Zhang; Xinqiu Tan; Yong Liu; Xuguo Zhou

doi:10.3390/insects12020101

Tomato chlorosis virus infection facilitates bemisia tabaci med reproduction by elevating vitellogenin expression

Liping Huang, Xiaobin Shi, Jizhe Shi, Zhuo Zhang, Yong Fang, Zhanhong Zhang, Qiuyi Pan, Limin Zheng, Yang Gao, Deyong Zhang, Xinqiu Tan, Yong Liu, Xuguo Zhou

Entomology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Transmission of plant pathogenic viruses mostly relies on insect vectors. Plant virus could enhance its transmission by modulating the vector. Previously, we showed that feeding on virus infected plants can promote the reproduction of the sweet potato whitefly, Bemisia tabaci MED (Q biotype). In this study, using a whitefly-Tomato chlorosis virus (ToCV)-tomato system, we investigated how ToCV modulates B. tabaci MED reproduction to facilitate its spread. Here, we hypothesized that ToCV-infected tomato plants would increase B. tabaci MED fecundity via elevated vitellogenin (Vg) gene expression. As a result, fecundity and the relative expression of B. tabaci MED Vg was measured on ToCV-infected and uninfected tomato plants on days 4, 8, 12, 16, 20 and 24. The role of Vg on B. tabaci MED reproduction was examined in the presence and absence of ToCV using dietary RNAi. ToCV infection significantly increased B. tabaci MED fecundity on days 12, 16 and 20, and elevated Vg expression on days 8, 12 and 16. Both ovarian development and fecundity of B. tabaci MED were suppressed when Vg was silenced with or without ToCV infection. These combined results suggest that ToCV infection increases B. tabaci MED fecundity via elevated Vg expression.

Original language	English
Article number	101
Pages (from-to)	1-12
Number of pages	12
Journal	Insects
Volume	12
Issue number	2
DOIs	https://doi.org/10.3390/insects12020101
State	Published - Feb 2021

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (no. 31872932, 31972242, 31672003), the Agriculture Research System of China (CARS-25-B-05), and Natural Science Foundation of Hunan Province (2019JJ30014). We thank Tao Zhou (China Agricultural University) for kindly providing the infectious cDNA clone of ToCV. Authors are also grateful to anonymous reviewers for their constructive comments and suggestions to improve this manuscript.

Funding Information:
Funding: This work was supported by the National Natural Science Foundation of China (no. 31872932, 31972242, 31672003), the Agriculture Research System of China (CARS-25-B-05), and Natural Science Foundation of Hunan Province (2019JJ30014).

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Bemisia tabaci
Fecundity
Gene expression
Ovarian development
RNA interference
Vitellogenin

ASJC Scopus subject areas

Insect Science

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10.3390/insects12020101

Cite this

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title = "Tomato chlorosis virus infection facilitates bemisia tabaci med reproduction by elevating vitellogenin expression",

abstract = "Transmission of plant pathogenic viruses mostly relies on insect vectors. Plant virus could enhance its transmission by modulating the vector. Previously, we showed that feeding on virus infected plants can promote the reproduction of the sweet potato whitefly, Bemisia tabaci MED (Q biotype). In this study, using a whitefly-Tomato chlorosis virus (ToCV)-tomato system, we investigated how ToCV modulates B. tabaci MED reproduction to facilitate its spread. Here, we hypothesized that ToCV-infected tomato plants would increase B. tabaci MED fecundity via elevated vitellogenin (Vg) gene expression. As a result, fecundity and the relative expression of B. tabaci MED Vg was measured on ToCV-infected and uninfected tomato plants on days 4, 8, 12, 16, 20 and 24. The role of Vg on B. tabaci MED reproduction was examined in the presence and absence of ToCV using dietary RNAi. ToCV infection significantly increased B. tabaci MED fecundity on days 12, 16 and 20, and elevated Vg expression on days 8, 12 and 16. Both ovarian development and fecundity of B. tabaci MED were suppressed when Vg was silenced with or without ToCV infection. These combined results suggest that ToCV infection increases B. tabaci MED fecundity via elevated Vg expression.",

keywords = "Bemisia tabaci, Fecundity, Gene expression, Ovarian development, RNA interference, Vitellogenin",

author = "Liping Huang and Xiaobin Shi and Jizhe Shi and Zhuo Zhang and Yong Fang and Zhanhong Zhang and Qiuyi Pan and Limin Zheng and Yang Gao and Deyong Zhang and Xinqiu Tan and Yong Liu and Xuguo Zhou",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (no. 31872932, 31972242, 31672003), the Agriculture Research System of China (CARS-25-B-05), and Natural Science Foundation of Hunan Province (2019JJ30014). We thank Tao Zhou (China Agricultural University) for kindly providing the infectious cDNA clone of ToCV. Authors are also grateful to anonymous reviewers for their constructive comments and suggestions to improve this manuscript. Funding Information: Funding: This work was supported by the National Natural Science Foundation of China (no. 31872932, 31972242, 31672003), the Agriculture Research System of China (CARS-25-B-05), and Natural Science Foundation of Hunan Province (2019JJ30014). Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

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doi = "10.3390/insects12020101",

language = "English",

volume = "12",

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T1 - Tomato chlorosis virus infection facilitates bemisia tabaci med reproduction by elevating vitellogenin expression

AU - Huang, Liping

AU - Shi, Xiaobin

AU - Shi, Jizhe

AU - Zhang, Zhuo

AU - Fang, Yong

AU - Zhang, Zhanhong

AU - Pan, Qiuyi

AU - Zheng, Limin

AU - Gao, Yang

AU - Zhang, Deyong

AU - Tan, Xinqiu

AU - Liu, Yong

AU - Zhou, Xuguo

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (no. 31872932, 31972242, 31672003), the Agriculture Research System of China (CARS-25-B-05), and Natural Science Foundation of Hunan Province (2019JJ30014). We thank Tao Zhou (China Agricultural University) for kindly providing the infectious cDNA clone of ToCV. Authors are also grateful to anonymous reviewers for their constructive comments and suggestions to improve this manuscript. Funding Information: Funding: This work was supported by the National Natural Science Foundation of China (no. 31872932, 31972242, 31672003), the Agriculture Research System of China (CARS-25-B-05), and Natural Science Foundation of Hunan Province (2019JJ30014). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/2

Y1 - 2021/2

N2 - Transmission of plant pathogenic viruses mostly relies on insect vectors. Plant virus could enhance its transmission by modulating the vector. Previously, we showed that feeding on virus infected plants can promote the reproduction of the sweet potato whitefly, Bemisia tabaci MED (Q biotype). In this study, using a whitefly-Tomato chlorosis virus (ToCV)-tomato system, we investigated how ToCV modulates B. tabaci MED reproduction to facilitate its spread. Here, we hypothesized that ToCV-infected tomato plants would increase B. tabaci MED fecundity via elevated vitellogenin (Vg) gene expression. As a result, fecundity and the relative expression of B. tabaci MED Vg was measured on ToCV-infected and uninfected tomato plants on days 4, 8, 12, 16, 20 and 24. The role of Vg on B. tabaci MED reproduction was examined in the presence and absence of ToCV using dietary RNAi. ToCV infection significantly increased B. tabaci MED fecundity on days 12, 16 and 20, and elevated Vg expression on days 8, 12 and 16. Both ovarian development and fecundity of B. tabaci MED were suppressed when Vg was silenced with or without ToCV infection. These combined results suggest that ToCV infection increases B. tabaci MED fecundity via elevated Vg expression.

AB - Transmission of plant pathogenic viruses mostly relies on insect vectors. Plant virus could enhance its transmission by modulating the vector. Previously, we showed that feeding on virus infected plants can promote the reproduction of the sweet potato whitefly, Bemisia tabaci MED (Q biotype). In this study, using a whitefly-Tomato chlorosis virus (ToCV)-tomato system, we investigated how ToCV modulates B. tabaci MED reproduction to facilitate its spread. Here, we hypothesized that ToCV-infected tomato plants would increase B. tabaci MED fecundity via elevated vitellogenin (Vg) gene expression. As a result, fecundity and the relative expression of B. tabaci MED Vg was measured on ToCV-infected and uninfected tomato plants on days 4, 8, 12, 16, 20 and 24. The role of Vg on B. tabaci MED reproduction was examined in the presence and absence of ToCV using dietary RNAi. ToCV infection significantly increased B. tabaci MED fecundity on days 12, 16 and 20, and elevated Vg expression on days 8, 12 and 16. Both ovarian development and fecundity of B. tabaci MED were suppressed when Vg was silenced with or without ToCV infection. These combined results suggest that ToCV infection increases B. tabaci MED fecundity via elevated Vg expression.

KW - Bemisia tabaci

KW - Fecundity

KW - Gene expression

KW - Ovarian development

KW - RNA interference

KW - Vitellogenin

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Tomato chlorosis virus infection facilitates bemisia tabaci med reproduction by elevating vitellogenin expression

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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