BACKGROUND: Plant viruses can facilitate their transmission by modulating the sex ratios of their insect vectors. Previously, we found that exposure to tomato spotted wilt orthotospovirus (TSWV) in the western flower thrips, Frankliniella occidentalis, led to a male-biased sex ratio in the offspring. TSWV, a generalist pathogen with a broad host range, is transmitted primarily by F. occidentalis in a circulative-propagative manner. Here, we integrated proteomic tools with RNAi to comprehensively investigate the genetic basis underlying the shift in vector sex ratio induced by the virus. RESULTS: Proteomic analysis exhibited 104 differentially expressed proteins between F. occidentalis adult males with and without TSWV. The expression of the fiber sheath CABYR-binding-like (FSCB) protein, namely FoFSCB-like, a sperm-specific protein associated with sperm capacitation and motility, was decreased by 46%. The predicted FoFSCB-like protein includes 10 classic Pro-X-X-Pro motifs and 42 phosphorylation sites, which are key features for sperm capacitation. FoFSCB-like expression was gradually increased during the development and peaked at the pupal stage. After exposure to TSWV, FoFSCB-like expression was substantially down-regulated. Nanoparticle-mediated RNAi substantially suppressed FoFSCB-like expression and led to a significant male bias in the offspring. CONCLUSION: These combined results suggest that down-regulation of FoFSCB-like in virus-exposed thrips leads to a male-biased sex ratio in the offspring. This study not only advances our understanding of virus-vector interactions, but also identifies a potential target for the genetic management of F. occidentalis, the primary vector of TSWV, by manipulating male fertility.
|Number of pages||10|
|Journal||Pest Management Science|
|State||Published - Nov 2022|
Bibliographical noteFunding Information:
This study was funded by China Agriculture Research System (CARS‐24‐ C‐02), the Natural Science Foundation of China (31572037), the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS‐ASTIP‐IVFCAAS), and the China Postdoctoral Science Foundation (2020 M670552).
This study was funded by China Agriculture Research System (CARS-24- C-02), the Natural Science Foundation of China (31572037), the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS), and the China Postdoctoral Science Foundation (2020 M670552).
© 2022 Society of Chemical Industry.
- Frankliniella occidentalis
- male sterility
- sex ratio
ASJC Scopus subject areas
- Agronomy and Crop Science
- Insect Science